- Actin
- Myosin
- Dynamin
- Tropomyosin
- Cell motility and cytoskeletal dynamics
- Intracellular pathogen sensors
- Protein structure, dynamics, reaction mechanisms, and allostery
- Small-molecule based approaches targeting myosin function
- Pharmacological chaperone-based approaches to treat muscle disorders and cytoskeletal protein-linked diseases
- Bacterial and eukaryotic protein expression (E.coli; D. discoideum; Spodoptera frugiperda)
- Protein purification (affinity-, ion-exchange, hydrophobic interaction, size-exclusion chromatography)
- Time-resolved spectroscopy
- Transient kinetics (stopped-flow, quenched-flow, flash photolysis of caged compounds, IR-laser induced temperature-jump)
- Circular dichroism spectroscopy (CD)
- Calorimetry (DSC and ITC)
- Dynamic light scattering (DLS)
- Microscale thermophoresis (MST)
- Analytical ultracentrifugation techniques (sedimentation velocity AUC; sedimentation equilibrium AUC)
- Differential scanning fluorescence (Thermo-Fluor assay)
- Single molecule and live-cell imaging approaches
- Confocal, evanescent field and spinning disk fluorescence microscopy
- Macromolecular X-ray crystallography (crystallization robots, crystal imagers, X-ray generators & detectors)
- Bioinformatics for integrated structural biology and hybrid methods
- Computer-assisted drug-design (3D-workstations, High-Performance Computing Cluster)