• Modulatory roles of sub-components of motor proteins
• ​Designing new nano-biohybrid motors based on natural building blocks
• ​Single molecule analysis of mutant myosin motors implicated in human hypertrophic cardiomyopathy
• ​Epigenetic regulation of straited muscle physiology by ubiquitin-like proteins

We employ a diversed array of cutting-edge experimental approaches.

For in-depth analysis motor protein function, we mainly use single molecule biophysical methods such as Total Internal Reflection Fluorescence microscopy (TIRF-M), optical trapping and zero mode waveguides to obtain precise kinetic and mechanical insights of the motor proteins.

​For our projects involving epigenetic regulation of muscle physiology, we use various biochemical and molecular biological tools such as in vivo Co-IP, protein network analysis by quantitative mass spectrometry, gene-expression array system, chromatin immunoprecipitation and ChIPseq etc.

Selected publications

• Single-Molecule Investigation of Load-Dependent Actomyosin Dissociation Kinetics for Cardiac and Slow Skeletal Myosin, Wang T, Nayak A, Kraft T, Amrute-Nayak M., Nano Micro Small. 2024 Oct 7:e2406865.
  doi: 10.1002/smll.202406865. Online ahead of print., Pubmed, Journal Link

• Sorafenib induces cachexia by impeding transcriptional signaling of the SET1/MLL complex on muscle-specific genes, 
  Bushra Khan, Chiara Lanzuolo, Valentina Rosti, Philina Santarelli, Andreas Pich,Theresia Kraft, Mamta Amrute-Nayak, Arnab Nayak, iScience . 2024 Sep 10;27(10):110913. eCollection 2024 Oct 18, https://doi.org/10.1016/j.isci.2024.110913, Link Journal, PubMed

• PyChelator: a Python-based Colab and web application for metal chelator calculations, Emrulla Spahiu, Esra Kastrati & Mamta Amrute-Nayak,  BMC Bioinformatics volume 25, Article number: 239 (2024), DOI: 10.1186/s12859-024-05858-8, Pubmed

• Cardiac ventricular myosin and slow skeletal myosin exhibit dissimilar chemomechanical properties despite bearing the same myosin heavy chain isoform, Tianbang Wang, Emrulla Spahiu, Jennifer Osten, Florentine Behrens, Fabius Grünhagen, Tim Scholz, Theresia Kraft, Arnab Nayak, Mamta Amrute-Nayak J Biol Chem . 2022 May 24;102070.  doi: 10.1016/j.jbc.2022.102070. [Online ahead of print] PubMed
   
• Acto-Myosin Cross-Bridge Stiffness Depends on the Nucleotide State of Myosin II. Wang T, Brenner B, Nayak A, and Amrute-Nayak M. Nano Letters, 2020 Sep 15. doi: 10.1021/acs.nanolett.0c02960. [Online ahead of print] PubMed
   
• Transformation of the Nonprocessive Fast Skeletal Myosin II into a Processive Motor. Amrute-Nayak M*, Nayak A, Steffen W, Tsiavaliaris G, Scholz T, Brenner B. Small. 2019 Jan 18:e1804313. doi: 10.1002/smll.201804313. (* - Corresponding author, IF - 9.598); PubMed

• ​MARK4 regulates NLRP3 positioning and inflammasome activation through a microtubule-dependent mechanism. Li X, Thome S, Ma X, Amrute-Nayak M, Finigan A, Kitt L, Masters L, James JR, Shi Y, Meng G, Mallat Z. Nat Commun. 2017 Jun 28. (IF - 12.353)

• ATP turnover by individual myosin molecules hints at two conformers of the myosin active site. Amrute-Nayak M, Lambeck KA, Radocaj A, Huhnt HE, Scholz T, Hahn N, Tsiavaliaris G, Walter WJ, Brenner B. Proc Natl Acad Sci U S A. 2014 Feb 18. (IF - 9.504)

• Single-molecule assays reveal that RNA localization signals regulate dynein-dynactin copy number on individual transcript cargoes. Amrute-Nayak M, Bullock SL. Nat Cell Biol. 2012 Feb 26. (IF - 20.06)

• Targeted optimization of a protein nanomachine for operation in biohybrid devices. Amrute-Nayak M, Diensthuber RP, Steffen W, Kathmann D, Hartmann FK, Fedorov R, Urbanke C, Manstein DJ, Brenner B, Tsiavaliaris G. Angew Chem Int Ed Engl. 2010. (IF - 12.10)

For my Masters (M. Sc.), I studied Biochemistry from Pune University, India.

For my PhD, I joined Prof Bernhard Brenner’s research lab at Medical School Hannover (MHH), Germany. Here I undertook a project that involved studying molecular motors using single-molecule fluorescence detection technique, Total internal reflection fluorescence microscopy.

For my post-doctoral research, I moved to Medical Research Council-Laboratory of Molecular Biology (MRC-LMB), Cambridge, UK to work with Dr. Simon Bullock.  I investigated the molecular mechanism of cytoplasmic mRNA transport. We attempted to dissect the interaction of localization signals with the microtubule-based motors that guide the mRNA through complex cytoskeletal network and sort different mRNAs.

Since 2017, I have established my research group at Medical school Hannover, Germany. The main focus of my lab is to investigate the molecular mechanisms underlying motor protein function. Furthermore, we investigate the naturally occurring mutations in sarcomereic proteins implicated in hypertrophic cardiomyopathy (HCM) in humans.

Contact Dr. Mamta Amrute

Dr. Tianbang Wang finished his doctoral studies in the research group of PD. Dr. Walter Steffen, Institute for Molecular-and Cell physiology, Hannover Medical School. Here he undertook the project about ‘Investigation of the force generation mechanism of cytoplasmic dynein’.

Since July 2018, He is working as a postdoctoral researcher in the research group of Dr. Mamta Amrute-Nayak, Institute for Molecular-and Cell physiology, Hannover Medical School

His project involved:

1. Characterizing the mechanical and kinetic properties of β-cardiac and skeletal muscle myosin II
2. Investigation of human ventricular myosin mutations implicated in human hypertrophic cardiomyopathy
3. Development of optical trap technique

Publications:

• Acto-Myosin Cross-Bridge Stiffness Depends on the Nucleotide State of Myosin II. Wang T, Brenner B, Nayak A, and Amrute-Nayak M. Nano Letters, 2020 Sep 15. doi: 10.1021/acs.nanolett.0c02960. [Online ahead of print] PubMed
   
• Single-molecule analysis reveals that regulatory light chains fine-tune skeletal myosin-II function, Nayak A, Wang T, Franz P, Steffen W, Chizhov I, Tsiavaliaris G, Amrute-Nayak M, J Biol Chem. 2020 Apr 9. pii: jbc.RA120.012774. doi: 10.1074/jbc.RA120.012774, PMID: 32273340, Artikel, PubMed
   
• Increased Cell-Matrix Adhesion upon Constitutive Activation of Rho Proteins by Cytotoxic Necrotizing Factors from E. coli and Y. pseudotuberculosis. Martin May, Tanja Kolbe, Tianbang Wang, Gudula Schmidt, and Harald Genth. J Signal Transduct. 2012;2012:570183.
   
• Difference in F-actin depolymerization induced by toxin B from the Clostridium difficile strain VPI 10463 and toxin B from the variant Clostridium difficile serotype F strain 1470.  May M, Wang T, Müller M, Genth H. Toxins (Basel). 2013 Jan 11;5(1):106-19.
   
• Mg2+-free ATP Regulates Native Cytoplasmic Dynein’s Processivity, VA Behrens, WJ Walter, C Peters, T Wang, B Brenner1, MA Geeves4, T Scholz1,5, W Steffen. FEBS Lett. 2018 Dec 21..
• The force generation of cytoplasmic dynein is load dependent. T Wang, WJ Walter, VA Behrens, W Steffen. Manuscript in preparation.

Contact Dr. Tianbang Wang

I finished my Master studies in Biology from Middle East Technical University (METU), Ankara, Turkey.

Since October 2020, I joined research group of Dr. Mamta Amrute-Nayak at the Institute for Molecular-and Cell physiology to pursue my doctoral studies. My project involves studying the cardiac myosin II mutations linked to cardiomyopathies using single-molecule investigation methods.

Medizinischen Technologin für Laboratoriumsanalytik (MTLA/MTA)

Ayesha completed her Bachelors studies (B.Sc.) in Microbiology from Government College University (GCU), Lahore, Pakistan. Later, she embarked on Masters studies (M.Sc) in Biology from Lahore University of Management Sciences (LUMS), Pakistan. 

In August 2022, Ayesha joined the research group of Dr. Mamta Amrute-Nayak at the Institute for Molecular and Cell physiology to pursue her doctoral studies. Her project involves the investigation of molecular mechanisms underlying cardiac dysfunction in Hypertrophic Cardiomyopathy. 

• Fabius Grünhagen
  Research team member as a FWJ (Freiwilliges Wissenschaftliches Jahr) student from October 2018 - August 2019. Fabius is currently pursuing his bachelor studies in Physics at Zurich University.
• Khalaf Rasho
  scientific assistant

• Florentine Behrens
  is a dentistry student currently pursuing her doctoral studies in the research group of Dr. Mamta Amrute-Nayak. Florentine is investigating the dysfunction of cardiac myosin motor due to single point mutations in the motor subcomponent, known to cause hypertrophy cardiomyopathy (HCM) in humans.