The Central Research Facility Proteomics

Core Facility Proteomics

The Proteomics lab is equipped to perform MS-based Proteomics using DDA and DIA methods and targeted protein analysis (mass western).

Contact

Prof. Dr. Andreas Pich

Tel.: 0511-532-2808

Karsten Heidrich

Ulrike Schrameck

Lea Flemming

Johanna Sophie Libnow

Jule Hupfeld

Tel.: 0511-532-5614

Tel.: 0511-532-174256

Equipment

Orbitrap Fusion Lumos (Thermo Fisher Scientific)

Orbitrap Exploris 240 (Thermo Fisher Scientific)

LTQ Orbitrap Velos (Thermo Fisher Scientific)

7500 QTRAP (Sciex)

5800 MALDI TOF/TOF (Sciex)

4 nanoLC-Systems

Agilent 1100 LC

For MS data analysis multi-core PCs and softwares like MaxQuant, Spectronaut, Proteome Discoverer, SciexOS and Skyline are available. Software tools like Perseus, Ingenuity Pathway Analysis can be used for bioinformatic analysis and we are familiar with R software and internet platforms like PantherDB, GSEA, and more.

Collaborations and services

The MS lab is open for collaborations and provides the following services:

• Protein and proteome analysis
• Phosphoproteomics
• Analysis of posttranslational modifications
• Targeted protein analysis (mass western)
• Consultations for proteome and protein analysis and the experimental design of MS-experiments

Instructions for protein identification

• Protein samples from nearly all sources can be analyzed. For proper identifications suitable data bases from e.g. Uniprot must be available.
• Proteins should be prepared in a suitable buffer or can be easily analyzed after SDS-PAGE. Please note that cysteine residues have to be alkylated before analysis. Methods for alkylation are available from the MS lab.