Publications

Publikationen LEBAO

in press

2024

 

Cubillos, P., Ditzer, N., Kolodziejczyk, A., Schwenk, G., Hoffmann, J., Schütze, T. M., Derihaci, R. P., Birdir, C., Köllner, J. E., Petzold, A., Sarov, M., Martin, U., Long, K. R., Wimberger, P., and Albert, M. 2024. The growth factor EPIREGULIN promotes basal progenitor cell proliferation in the developing neocortex. Embo j 43, no. 8:1388.

https://doi.org/10.1038/s44318-024-00068-7.

 

Goecke, T., Ius, F., Ruhparwar, A., and Martin, U. 2024. Unlocking the Future: Pluripotent Stem Cell-Based Lung Repair. Cells 13, no. 7.

https://doi.org/10.3390/cells13070635.

 

2023

 

Bojkova, D., Bechtel, M., Rothenburger, T., Kandler, J. D., Hayes, L., Olmer, R., Martin, U., Jonigk, D., Ciesek, S., Wass, M. N., Michaelis, M., and Cinatl, J., Jr. 2023. Omicron-induced interferon signaling prevents influenza A H1N1 and H5N1 virus infection. J Med Virol 95, no. 3:e28686.

https://doi.org/10.1002/jmv.28686.

 

Bojkova, D., Reus, P., Panosch, L., Bechtel, M., Rothenburger, T., Kandler, J. D., Pfeiffer, A., Wagner, J. U. G., Shumliakivska, M., Dimmeler, S., Olmer, R., Martin, U., Vondran, F. W. R., Toptan, T., Rothweiler, F., Zehner, R., Rabenau, H. F., Osman, K. L., Pullan, S. T., Carroll, M. W., Stack, R., Ciesek, S., Wass, M. N., Michaelis, M., and Cinatl, J., Jr. 2023. Identification of novel antiviral drug candidates using an optimized SARS-CoV-2 phenotypic screening platform. iScience 26, no. 2:105944.

https://doi.org/10.1016/j.isci.2023.105944.

 

Drakhlis, L., and Zweigerdt, R. 2023. Heart in a dish - choosing the right in vitro model. Dis Model Mech 16, no. 5.

https://doi.org/10.1242/dmm.049961.

 

Eiringhaus, J., de Vries, A. L., Hohmann, S., Böthig, D., Müller-Leisse, J., Hillmann, H. A. K., Martens, A., Zweigerdt, R., Schrod, A., Martin, U., Duncker, D., Gruh, I., and Veltmann, C. 2023. Performance and feasibility of three different approaches for computer based semi-automated analysis of ventricular arrhythmias in telemetric long-term ECG in cynomolgus monkeys. J Pharmacol Toxicol Methods 124:107471.

https://doi.org/10.1016/j.vascn.2023.107471.

 

Harrison, S. P., Siller, R., Tanaka, Y., Chollet, M. E., de la Morena-Barrio, M. E., Xiang, Y., Patterson, B., Andersen, E., Bravo-Pérez, C., Kempf, H., Åsrud, K. S., Lunov, O., Dejneka, A., Mowinckel, M. C., Stavik, B., Sandset, P. M., Melum, E., Baumgarten, S., Bonanini, F., Kurek, D., Mathapati, S., Almaas, R., Sharma, K., Wilson, S. R., Skottvoll, F. S., Boger, I. C., Bogen, I. L., Nyman, T. A., Wu, J. J., Bezrouk, A., Cizkova, D., Corral, J., Mokry, J., Zweigerdt, R., Park, I. H., and Sullivan, G. J. 2023. Scalable production of tissue-like vascularized liver organoids from human PSCs. Exp Mol Med 55, no. 9:2005.

https://doi.org/10.1038/s12276-023-01074-1.

 

Heseding, H., Jahn, K., Brändl, B., Haase, A., Shum, I. O., Kohrn, T., Bleich, S., Frieling, H., Martin, U., Müller, F. J., Wunderlich, S., and Deest, M. 2023. Generation of an induced pluripotent stem cell line, ZIPi021-A, from fibroblasts of a Prader-Willi syndrome patient with maternal uniparental disomy (mUPD). Stem Cell Res 71:103143.

https://doi.org/10.1016/j.scr.2023.103143.

 

Junge, S., Ricci Signorini, M. E., Al Masri, M., Gülink, J., Brüning, H., Kasperek, L., Szepes, M., Bakar, M., Gruh, I., Heisterkamp, A., and Torres-Mapa, M. L. 2023. A micro-LED array based platform for spatio-temporal optogenetic control of various cardiac models. Sci Rep 13, no. 1:19490.

https://doi.org/10.1038/s41598-023-46149-1.

 

Lin, C., Kuffour, E. O., Fuchs, N. V., Gertzen, C. G. W., Kaiser, J., Hirschenberger, M., Tang, X., Xu, H. C., Michel, O., Tao, R., Haase, A., Martin, U., Kurz, T., Drexler, I., Görg, B., Lang, P. A., Luedde, T., Sparrer, K. M. J., Gohlke, H., König, R., and Münk, C. 2023. Regulation of STING activity in DNA sensing by ISG15 modification. Cell Rep 42, no. 11:113277.

https://doi.org/10.1016/j.celrep.2023.113277.

 

Merkert, S., Haase, A., Dahlmann, J., Göhring, G., Waqas, F. H., Pessler, F., Martin, U.#, and Olmer, R.#. 2023. Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing. Stem Cell Res 69:103090.

https://doi.org/10.1016/j.scr.2023.103090.

 

Mhlekude, B., Postmus, D., Stenzel, S., Weiner, J., 3rd, Jansen, J., Zapatero-Belinchón, F. J., Olmer, R., Richter, A., Heinze, J., Heinemann, N., Mühlemann, B., Schroeder, S., Jones, T. C., Müller, M. A., Drosten, C., Pich, A., Thiel, V., Martin, U., Niemeyer, D., Gerold, G., Beule, D., and Goffinet, C. 2023. Pharmacological inhibition of bromodomain and extra-terminal proteins induces an NRF-2-mediated antiviral state that is subverted by SARS-CoV-2 infection. PLoS Pathog 19, no. 9:e1011657.

https://doi.org/10.1371/journal.ppat.1011657.

 

Ori, C., Ansari, M., Angelidis, I., Olmer, R., Martin, U., Theis, F. J., Schiller, H. B., and Drukker, M. 2023. Human pluripotent stem cell fate trajectories toward lung and hepatocyte progenitors. iScience 26, no. 11:108205.

https://doi.org/10.1016/j.isci.2023.108205.

 

Osten, F., Weber, N., Wendland, M., Holler, T., Piep, B., Kröhn, S., Teske, J., Bodenschatz, A. K., Devadas, S. B., Menge, K. S., Chatterjee, S., Schwanke, K., Kosanke, M., Montag, J., Thum, T., Zweigerdt, R., Kraft, T., Iorga, B., and Meissner, J. D. 2023. Myosin expression and contractile function are altered by replating stem cell-derived cardiomyocytes. J Gen Physiol 155, no. 11.

https://doi.org/10.1085/jgp.202313377.

 

Shum, I. O., Merkert, S., Malysheva, S., Jahn, K., Lachmann, N., Verboom, M., Frieling, H., Hallensleben, M., and Martin, U. 2023. An Improved Protocol for Targeted Differentiation of Primed Human Induced Pluripotent Stem Cells into HLA-G-Expressing Trophoblasts to Enable the Modeling of Placenta-Related Disorders. Cells 12, no. 16.

https://doi.org/10.3390/cells12162070.

 

Staar, B. O., Hegermann, J., Auber, B., Ewen, R., von Hardenberg, S., Olmer, R., Pink, I., Rademacher, J., Wetzke, M., and Ringshausen, F. C. 2023. Ciliary Ultrastructure Assessed by Transmission Electron Microscopy in Adults with Bronchiectasis and Suspected Primary Ciliary Dyskinesia but Inconclusive Genotype. Cells 12, no. 22.

https://doi.org/10.3390/cells12222651.

 

Vivekanandan, R., Szepes, M., Ricci Signorini, M. E., Kravchenko, D., Kiefer, J., Berger, S., Fricke, V., Göhring, G., and Gruh, I. 2023. Generation of human induced pluripotent stem cell line encoding for a genetically encoded voltage indicator Arclight A242. Stem Cell Res 66:102981.

https://doi.org/10.1016/j.scr.2022.102981.

 

von Schledorn, L., Puertollano Martín, D., Cleve, N., Zöllner, J., Roth, D., Staar, B. O., Hegermann, J., Ringshausen, F. C., Nawroth, J., Martin, U.#, and Olmer, R.#. 2023. Primary Ciliary Dyskinesia Patient-Specific hiPSC-Derived Airway Epithelium in Air-Liquid Interface Culture Recapitulates Disease Specific Phenotypes In Vitro. Cells 12, no. 11. # authors contributed equally.

https://doi.org/10.3390/cells12111467.

 

Waqas, F. H., Shehata, M., Elgaher, W. A. M., Lacour, A., Kurmasheva, N., Begnini, F., Kiib, A. E., Dahlmann, J., Chen, C., Pavlou, A., Poulsen, T. B., Merkert, S., Martin, U., Olmer, R., Olagnier, D., Hirsch, A. K. H., Pleschka, S., and Pessler, F. 2023. NRF2 activators inhibit influenza A virus replication by interfering with nucleo-cytoplasmic export of viral RNPs in an NRF2-independent manner. PLoS Pathog 19, no. 7:e1011506. * authors contributed equally.

https://doi.org/10.1371/journal.ppat.1011506.

 

2022

 

Ackermann, M., Rafiei Hashtchin, A., Manstein, F., Carvalho Oliveira, M., Kempf, H., Zweigerdt, R., and Lachmann, N. 2022. Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors. Nat Protoc 17, no. 2:513.

https://doi.org/10.1038/s41596-021-00654-7.

 

Gonzalez, D. M., Schrode, N., Ebrahim, T. A. M., Broguiere, N., Rossi, G., Drakhlis, L., Zweigerdt, R., Lutolf, M. P., Beaumont, K. G., Sebra, R., and Dubois, N. C. 2022. Dissecting mechanisms of chamber-specific cardiac differentiation and its perturbation following retinoic acid exposure. Development 149, no. 13.

https://doi.org/10.1242/dev.200557.

 

Jaboreck, M. C., Lühmann, J. L., Mielenz, M., Stanke, F., Göhring, G., Martin, U., Olmer, R.#, and Merkert, S.#. 2022. Generation of two TMEM16A knockout iPSC clones each from a healthy human iPSC line, from a Cystic Fibrosis patient specific line with p.Phe508del mutation and from the gene corrected iPSC line. Stem Cell Res 64:102918. #authors contributed equally.

https://doi.org/10.1016/j.scr.2022.102918.

 

Jaeger, B., Schupp, J. C., Plappert, L., Terwolbeck, O., Artysh, N., Kayser, G., Engelhard, P., Adams, T. S., Zweigerdt, R., Kempf, H., Lienenklaus, S., Garrels, W., Nazarenko, I., Jonigk, D., Wygrecka, M., Klatt, D., Schambach, A., Kaminski, N., and Prasse, A. 2022. Airway basal cells show a dedifferentiated KRT17(high)Phenotype and promote fibrosis in idiopathic pulmonary fibrosis. Nat Commun 13, no. 1:5637.

https://doi.org/10.1038/s41467-022-33193-0.

 

Kirchhoff, H., Ricke-Hoch, M., Wohlan, K., Pietzsch, S., Karsli, Ü, Erschow, S., Zweigerdt, R., Ganser, A., Eder, M., Scherr, M., and Hilfiker-Kleiner, D. 2022. Chemotherapy-Free Targeted Anti-BCR-ABL+ Acute Lymphoblastic Leukemia Therapy May Benefit the Heart. Cancers (Basel) 14, no. 4.

https://doi.org/10.3390/cancers14040983.

 

Lindner, M., Laporte, A., Elomaa, L., Lee-Thedieck, C., Olmer, R., and Weinhart, M. 2022. Flow-induced glycocalyx formation and cell alignment of HUVECs compared to iPSC-derived ECs for tissue engineering applications. Front Cell Dev Biol 10:953062.

https://doi.org/10.3389/fcell.2022.953062.

 

Malik, M. N. H., Waqas, S. F., Zeitvogel, J., Cheng, J., Geffers, R., Gouda, Z. A., Elsaman, A. M., Radwan, A. R., Schefzyk, M., Braubach, P., Auber, B., Olmer, R., Müsken, M., Roesner, L. M., Gerold, G., Schuchardt, S., Merkert, S., Martin, U., Meissner, F., Werfel, T., and Pessler, F. 2022. Congenital deficiency reveals critical role of ISG15 in skin homeostasis. J Clin Invest 132, no. 3.

https://doi.org/10.1172/jci141573.

 

Niemeyer, D., Stenzel, S., Veith, T., Schroeder, S., Friedmann, K., Weege, F., Trimpert, J., Heinze, J., Richter, A., Jansen, J., Emanuel, J., Kazmierski, J., Pott, F., Jeworowski, L. M., Olmer, R., Jaboreck, M. C., Tenner, B., Papies, J., Walper, F., Schmidt, M. L., Heinemann, N., Möncke-Buchner, E., Baumgardt, M., Hoffmann, K., Widera, M., Thao, T. T. N., Balázs, A., Schulze, J., Mache, C., Jones, T. C., Morkel, M., Ciesek, S., Hanitsch, L. G., Mall, M. A., Hocke, A. C., Thiel, V., Osterrieder, K., Wolff, T., Martin, U., Corman, V. M., Müller, M. A., Goffinet, C., and Drosten, C. 2022. SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression. PLoS Biol 20, no. 11:e3001871.

https://doi.org/10.1371/journal.pbio.3001871.

 

Oripov, F., Ramm, R., Falk, C., Goecke, T., Ebken, J., Jashari, R., Böthig, D., Horke, A., Avsar, M., Bobylev, D., Haverich, A., Hilfiker, A., and Sarikouch, S. 2022. Serial assessment of early antibody binding to decellularized valved allografts. Front Cardiovasc Med 9:895943.

https://doi.org/10.3389/fcvm.2022.895943.

 

Ricci Signorini, M. E., Szepes, M., Melchert, A., Bakar, M., Merkert, S., Haase, A., Göhring, G., Martin, U., and Gruh, I. 2022. Generation of human induced pluripotent stem cell lines encoding for genetically encoded calcium indicators RCaMP1h and GCaMP6f. Stem Cell Res 60:102697.

https://doi.org/10.1016/j.scr.2022.102697.

 

Schilling, T., Meyer, T., Brandes, G., Hartung, D., Tudorache, I., Nolte, I., Wacker, F., Hilfiker, A., Hoeffler, K., Haverich, A., and Cebotari, S. 2022. Left Ventricular Wall Reconstruction with Autologous Vascularized Gastric Graft in a Porcine Pilot Model. Eur Surg Res.

https://doi.org/10.1159/000522478.

 

van der Velden, J., Asselbergs, F. W., Bakkers, J., Batkai, S., Bertrand, L., Bezzina, C. R., Bot, I., Brundel, Bjjm, Carrier, L., Chamuleau, S., Ciccarelli, M., Dawson, D., Davidson, S. M., Dendorfer, A., Duncker, D. J., Eschenhagen, T., Fabritz, L., Falcão-Pires, I., Ferdinandy, P., Giacca, M., Girao, H., Gollmann-Tepeköylü, C., Gyongyosi, M., Guzik, T. J., Hamdani, N., Heymans, S., Hilfiker, A., Hilfiker-Kleiner, D., Hoekstra, A. G., Hulot, J. S., Kuster, D. W. D., van Laake, L. W., Lecour, S., Leiner, T., Linke, W. A., Lumens, J., Lutgens, E., Madonna, R., Maegdefessel, L., Mayr, M., van der Meer, P., Passier, R., Perbellini, F., Perrino, C., Pesce, M., Priori, S., Remme, C. A., Rosenhahn, B., Schotten, U., Schulz, R., Sipido, K. R., Sluijter, J. P. G., van Steenbeek, F., Steffens, S., Terracciano, C. M., Tocchetti, C. G., Vlasman, P., Yeung, K. K., Zacchigna, S., Zwaagman, D., and Thum, T. 2022. Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC Working Group on Myocardial Function and the ESC Working Group on Cellular Biology of the Heart. Cardiovasc Res 118, no. 15:3016.

https://doi.org/10.1093/cvr/cvab370.

 

Waqas, S. F., Sohail, A., Nguyen, A. H. H., Usman, A., Ludwig, T., Wegner, A., Malik, M. N. H., Schuchardt, S., Geffers, R., Winterhoff, M., Merkert, S., Martin, U., Olmer, R., Lachmann, N., and Pessler, F. 2022. ISG15 deficiency features a complex cellular phenotype that responds to treatment with itaconate and derivatives. Clin Transl Med 12, no. 7:e931.

https://doi.org/10.1002/ctm2.931.

 

Wunderlich, S., Haase, A., Merkert, S., Jahn, K., Deest, M., Frieling, H., Glage, S., Korte, W., Martens, A., Kirschning, A., Zeug, A., Ponimaskin, E., Göhring, G., Ackermann, M., Lachmann, N., Moritz, T., Zweigerdt, R., and Martin, U. 2022. Targeted biallelic integration of an inducible Caspase 9 suicide gene in iPSCs for safer therapies. Mol Ther Methods Clin Dev 26:84.

https://doi.org/10.1016/j.omtm.2022.05.011.

 

2021

 

Andrade, A. C., Wolf, M., Binder, H. M., Gomes, F. G., Manstein, F., Ebner-Peking, P., Poupardin, R., Zweigerdt, R., Schallmoser, K., and Strunk, D. 2021. Hypoxic Conditions Promote the Angiogenic Potential of Human Induced Pluripotent Stem Cell-Derived Extracellular Vesicles. Int J Mol Sci 22, no. 8.

https://doi.org/10.3390/ijms22083890.

 

Banerjee, S., Szepes, M., Dibbert, N., Rios-Camacho, J. C., Kirschning, A., Gruh, I., and Dräger, G. 2021. Dextran-based scaffolds for in-situ hydrogelation: Use for next generation of bioartificial cardiac tissues. Carbohydr Polym 262:117924.

https://doi.org/10.1016/j.carbpol.2021.117924.

 

Benito-Kwiecinski, S.*, Giandomenico, S. L.*, Sutcliffe, M., Riis, E. S., Freire-Pritchett, P., Kelava, I., Wunderlich, S., Martin, U., Wray, G. A., McDole, K., and Lancaster, M. A. 2021. An early cell shape transition drives evolutionary expansion of the human forebrain. Cell 184, no. 8:2084. * authors contributed equally.

https://doi.org/10.1016/j.cell.2021.02.050.

 

Chatterjee, S., Hofer, T., Costa, A., Lu, D., Batkai, S., Gupta, S. K., Bolesani, E., Zweigerdt, R., Megias, D., Streckfuss-Bömeke, K., Brandenberger, C., Thum, T., and Bär, C. 2021. Telomerase therapy attenuates cardiotoxic effects of doxorubicin. Mol Ther 29, no. 4:1395.

https://doi.org/10.1016/j.ymthe.2020.12.035.

 

Drakhlis, L., Biswanath, S., Farr, C. M., Lupanow, V., Teske, J., Ritzenhoff, K., Franke, A., Manstein, F., Bolesani, E., Kempf, H., Liebscher, S., Schenke-Layland, K., Hegermann, J., Nolte, L., Meyer, H., de la Roche, J., Thiemann, S., Wahl-Schott, C., Martin, U., and Zweigerdt, R. 2021. Human heart-forming organoids recapitulate early heart and foregut development. Nat Biotechnol 39, no. 6:737.

https://doi.org/10.1038/s41587-021-00815-9.

 

Drakhlis, L.*, Devadas, S. B.*, and Zweigerdt, R. 2021. Generation of heart-forming organoids from human pluripotent stem cells. Nat Protoc. * authors contributed equally.

https://doi.org/10.1038/s41596-021-00629-8.

 

Ebken, J., Mester, N., Smart, I., Ramm, R., Goecke, T., Jashari, R., Böthig, D., Horke, A., Cebotari, S., Tudorache, I., Avsar, M., Bobylev, D., Haverich, A., Sarikouch, S., and Hilfiker, A. 2021. Residual immune response towards decellularized homografts may be highly individual. Eur J Cardiothorac Surg 59, no. 4:773.

https://doi.org/10.1093/ejcts/ezaa393.

 

Eggenschwiler, R., Gschwendtberger, T., Felski, C., Jahn, C., Langer, F., Sterneckert, J., Hermann, A., Lühmann, J., Steinemann, D., Haase, A., Martin, U., Petri, S., and Cantz, T. 2021. A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines. Sci Rep 11, no. 1:22154.

https://doi.org/10.1038/s41598-021-01689-2.

 

Eyre, K., Samper, E., Haverich, A., Hilfiker, A., and Andrée, B. 2021. Re-endothelialization of non-detergent decellularized porcine vessels. Artif Organs 45, no. 4:E53.

https://doi.org/10.1111/aor.13836.

 

Haase, A., Kohrn, T., Fricke, V., Ricci Signorini, M. E., Witte, M., Göhring, G., Gruh, I., and Martin, U. 2021. Establishment of MHHi001-A-5, a GCaMP6f and RedStar dual reporter human iPSC line for in vitro and in vivo characterization and in situ tracing of iPSC derivatives. Stem Cell Res 52:102206.

https://doi.org/10.1016/j.scr.2021.102206.

 

Kosanke, M., Davenport, C., Szepes, M., Wiehlmann, L., Kohrn, T., Dorda, M., Gruber, J., Menge, K., Sievert, M., Melchert, A., Gruh, I., Göhring, G., and Martin, U. 2021. iPSC culture expansion selects against putatively actionable mutations in the mitochondrial genome. Stem Cell Reports 16, no. 10:2488.

https://doi.org/10.1016/j.stemcr.2021.08.016.

 

Kosanke, M., Osetek, K., Haase, A., Wiehlmann, L., Davenport, C., Schwarzer, A., Adams, F., Kleppa, M. J., Schambach, A., Merkert, S., Wunderlich, S., Menke, S., Dorda, M., and Martin, U. 2021. Reprogramming enriches for somatic cell clones with small-scale mutations in cancer-associated genes. Mol Ther.

https://doi.org/10.1016/j.ymthe.2021.04.007.

 

Liu, D., Caliskan, S., Rashidfarokhi, B., Oldenhof, H., Jung, K., Sieme, H., Hilfiker, A., and Wolkers, W. F. 2021. Fourier transform infrared spectroscopy coupled with machine learning classification for identification of oxidative damage in freeze-dried heart valves. Sci Rep 11, no. 1:12299.

https://doi.org/10.1038/s41598-021-91802-2.

 

Malik, M. N. H., Waqas, S. F. H., Zeitvogel, J., Cheng, J., Geffers, R., Gouda, Z. A., Elsaman, A. M., Radwan, A. R., Schefzyk, M., Braubach, P., Auber, B., Olmer, R., Müsken, M., Roesner, L. M., Gerold, G., Schuchardt, S., Merkert, S., Martin, U., Meissner, F., Werfel, T., and Pessler, F. 2021. Congenital deficiency reveals critical role of ISG15 in skin homeostasis. J Clin Invest.

https://doi.org/10.1172/jci141573.

 

Manstein, F., Ullmann, K., Kropp, C., Halloin, C., Triebert, W., Franke, A., Farr, C. M., Sahabian, A., Haase, A., Breitkreuz, Y., Peitz, M., Brüstle, O., Kalies, S., Martin, U., Olmer, R., and Zweigerdt, R. 2021. High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling. Stem Cells Transl Med.

https://doi.org/10.1002/sctm.20-0453.

 

Manstein, F., Ullmann, K., Triebert, W., and Zweigerdt, R. 2021. Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors. STAR Protoc 2, no. 4:100988.

https://doi.org/10.1016/j.xpro.2021.100988.

 

Merkert, S., Wunderlich, S., Beier, J., Franke, A., Schwanke, K., Göhring, G., Kraft, T., Francino, A., Zweigerdt, R., and Martin, U. 2021. Generation of two iPSC clones (MHHi021-A and MHHi021-B) from a patient with hypertrophic cardiomyopathy with p.Arg723Gly mutation in the MYH7 gene. Stem Cell Res 52:102208.

https://doi.org/10.1016/j.scr.2021.102208.

 

Morticelli, L., Magdei, M., Tschalaki, N., Petersen, B., Haverich, A., and Hilfiker, A. 2021. Generation of glycans depleted decellularized porcine pericardium, using digestive enzymatic supplements and enzymatic mixtures for food industry. Xenotransplantation:e12705.

https://doi.org/10.1111/xen.12705.

 

Müller, D., Donath, S., Brückner, E. G., Biswanath Devadas, S., Daniel, F., Gentemann, L., Zweigerdt, R., Heisterkamp, A., and Kalies, S. M. K. 2021. How Localized Z-Disc Damage Affects Force Generation and Gene Expression in Cardiomyocytes. Bioengineering (Basel) 8, no. 12.

https://doi.org/10.3390/bioengineering8120213.

 

Pflaum, M., Dahlmann, J., Engels, L., Naghilouy-Hidaji, H., Adam, D., Zöllner, J., Otto, A., Schmeckebier, S., Martin, U., Haverich, A., Olmer, R., and Wiegmann, B. 2021. Towards Biohybrid Lung: Induced Pluripotent Stem Cell Derived Endothelial Cells as Clinically Relevant Cell Source for Biologization. Micromachines (Basel) 12, no. 8.

https://doi.org/10.3390/mi12080981.

 

Polyak, A., Bankstahl, J. P., Besecke, K. F. W., Hozsa, C., Triebert, W., Pannem, R. R., Manstein, F., Borcholte, T., Furch, M., Zweigerdt, R., Gieseler, R. K., Bengel, F. M., and Ross, T. L. 2021. Simplified (89)Zr-Labeling Protocol of Oxine (8-Hydroxyquinoline) Enabling Prolonged Tracking of Liposome-Based Nanomedicines and Cells. Pharmaceutics 13, no. 7.

https://doi.org/10.3390/pharmaceutics13071097.

 

Rafiei Hashtchin, A., Fehlhaber, B., Hetzel, M., Manstein, F., Stalp, J. L., Glage, S., Abeln, M., Zweigerdt, R., Munder, A., Viemann, D., Ackermann, M., and Lachmann, N. 2021. Human iPSC-derived macrophages for efficient Staphylococcus aureus clearance in a murine pulmonary infection model. Blood Adv.

https://doi.org/10.1182/bloodadvances.2021004853.

 

Ricke-Hoch, M., Stelling, E., Lasswitz, L., Gunesch, A. P., Kasten, M., Zapatero-Belinchón, F. J., Brogden, G., Gerold, G., Pietschmann, T., Montiel, V., Balligand, J. L., Facciotti, F., Hirsch, E., Gausepohl, T., Elbahesh, H., Rimmelzwaan, G. F., Höfer, A., Kühnel, M. P., Jonigk, D., Eigendorf, J., Tegtbur, U., Mink, L., Scherr, M., Illig, T., Schambach, A., Pfeffer, T. J., Hilfiker, A., Haverich, A., and Hilfiker-Kleiner, D. 2021. Impaired immune response mediated by prostaglandin E2 promotes severe COVID-19 disease. PLoS One 16, no. 8:e0255335.

https://doi.org/10.1371/journal.pone.0255335.

 

Sahabian, A.*, Dahlmann, J.*, Martin, U.#, and Olmer, R.#. 2021. Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions. Nat Protoc. */# authors contributed equally.

https://doi.org/10.1038/s41596-020-00470-5.

 

Smart, I., Goecke, T., Ramm, R., Petersen, B., Lenz, D., Haverich, A., Niemann, H., and Hilfiker, A. 2021. Dot blots of solubilized extracellular matrix allow quantification of human antibodies bound to epitopes present in decellularized porcine pulmonary heart valves. Xenotransplantation 28, no. 1:e12646.

https://doi.org/10.1111/xen.12646.

 

Wolkers, W. F., and Hilfiker, A. 2021. Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability. Methods Mol Biol 2180:731.

https://doi.org/10.1007/978-1-0716-0783-1_40.

 

2020

 

Dahlmann, J., Sahabian, A., Drick, N., Haase, A., Göhring, G., Lachmann, N., Ringshausen, F. C., Welte, T., Martin, U., and Olmer, R. 2020. Generation of two hiPSC lines (MHHi016-A, MHHi016-B) from a primary ciliary dyskinesia patient carrying a homozygous 5 bp duplication (c.248_252dup (p.Gly85Cysfs*11)) in exon 1 of the CCNO gene. Stem Cell Res 46:101850.

https://doi.org/10.1016/j.scr.2020.101850.

 

Drick, N., Dahlmann, J., Sahabian, A., Haase, A., Göhring, G., Lachmann, N., Ringshausen, F. C., Welte, T., Martin, U., and Olmer, R. 2020. Generation of two human induced pluripotent stem cell lines (MHHi017-A, MHHi017-B) from a patient with primary ciliary dyskinesia carrying a homozygous mutation (c.7915C > T [p.Arg2639*]) in the DNAH5 gene. Stem Cell Res 46:101848.

https://doi.org/10.1016/j.scr.2020.101848.

 

Drick, N., Sahabian, A., Pongpamorn, P., Merkert, S., Göhring, G., Welte, T., Martin, U., and Olmer, R. 2020. Generation of a NKX2.1 - p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4). Stem Cell Res 42:101659.

https://doi.org/10.1016/j.scr.2019.101659.

 

Findeisen, K., Morticelli, L., Goecke, T., Kolbeck, L., Ramm, R., Höffler, H. K., Brandes, G., Korossis, S., Haverich, A., and Hilfiker, A. 2020. Toward acellular xenogeneic heart valve prostheses: Histological and biomechanical characterization of decellularized and enzymatically deglycosylated porcine pulmonary heart valve matrices. Xenotransplantation 27, no. 5:e12617.

https://doi.org/10.1111/xen.12617.

 

Godehardt, A. W., Ramm, R., Gulich, B., Tönjes, R. R., and Hilfiker, A. 2020. Decellularized pig pulmonary heart valves-Depletion of nucleic acids measured by proviral PERV pol. Xenotransplantation 27, no. 2:e12565.

https://doi.org/10.1111/xen.12565.

 

Hunkler, H. J., Hoepfner, J., Huang, C. K., Chatterjee, S., Jara-Avaca, M., Gruh, I., Bolesani, E., Zweigerdt, R., Thum, T., and Bär, C. 2020. The Long Non-coding RNA Cyrano Is Dispensable for Pluripotency of Murine and Human Pluripotent Stem Cells. Stem Cell Reports 15, no. 1:13.

https://doi.org/10.1016/j.stemcr.2020.05.011.

 

Ichanti, H., Sladic, S., Kalies, S., Haverich, A., Andrée, B., and Hilfiker, A. 2020. Characterization of Tissue Engineered Endothelial Cell Networks in Composite Collagen-Agarose Hydrogels. Gels 6, no. 3.

https://doi.org/10.3390/gels6030027.

 

Kreuser, U., Buchert, J., Haase, A., Richter, W., and Diederichs, S. 2020. Initial WNT/β-Catenin Activation Enhanced Mesoderm Commitment, Extracellular Matrix Expression, Cell Aggregation and Cartilage Tissue Yield From Induced Pluripotent Stem Cells. Front Cell Dev Biol 8:581331.

https://doi.org/10.3389/fcell.2020.581331.

 

Lipus, A.*, Janosz, E.*, Ackermann, M., Hetzel, M., Dahlke, J., Buchegger, T., Wunderlich, S., Martin, U., Cathomen, T., Schambach, A., Moritz, T.#, and Lachmann, N.#. 2020. Targeted Integration of Inducible Caspase-9 in Human iPSCs Allows Efficient in vitro Clearance of iPSCs and iPSC-Macrophages. Int J Mol Sci 21, no. 7. */# authors contributed equally.

https://doi.org/10.3390/ijms21072481.

 

Merkert, S., Jaboreck, M. C., Engels, L., Malik, M. N. H., Göhring, G., Pessler, F., Martin, U., and Olmer, R. 2020. Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing. Stem Cell Res 50:102135

https://doi.org/10.1016/j.scr.2020.102135.

 

Merkert, S., Schubert, M., Haase, A., Janssens, H. M., Scholte, B., Lachmann, N., Gohring, G., and Martin, U. 2020. Generation of an induced pluripotent stem cell line (MHHi018-A) from a patient with Cystic Fibrosis carrying p.Asn1303Lys (N1303K) mutation. Stem Cell Res 44:101744.

https://doi.org/10.1016/j.scr.2020.101744.

 

Nowak-Imialek, M., Wunderlich, S., Herrmann, D., Breitschuh-Leibling, S., Gohring, G., Petersen, B., Klein, S., Baulain, U., Lucas-Hahn, A., Martin, U., and Niemann, H. 2020. In Vitro and In Vivo Interspecies Chimera Assay Using Early Pig Embryos. Cell Reprogram 22, no. 3:118.

https://doi.org/10.1089/cell.2019.0107.

 

Pongpamorn, P.*, Dahlmann, J.*, Haase, A., Ebeling, C. T., Merkert, S., Gohring, G., Lachmann, N., Martens, A., Haverich, A., Martin, U.#, and Olmer, R. #. 2020. Generation of three induced pluripotent stem cell lines (MHHi012-A, MHHi013-A, MHHi014-A) from a family with Loeys-Dietz syndrome carrying a heterozygous p.M253I (c.759G>A) mutation in the TGFBR1 gene. Stem Cell Res 43:101707. */# authors contributed equally.

https://doi.org/10.1016/j.scr.2020.101707.

 

Ramm, R., Goecke, T., Theodoridis, K., Hoeffler, K., Sarikouch, S., Findeisen, K., Ciubotaru, A., Cebotari, S., Tudorache, I., Haverich, A., and Hilfiker, A. 2020. Decellularization combined with enzymatic removal of N-linked glycans and residual DNA reduces inflammatory response and improves performance of porcine xenogeneic pulmonary heart valves in an ovine in vivo model. Xenotransplantation 27, no. 2:e12571.

https://doi.org/10.1111/xen.12571.

 

Sahabian, A., von Schlehdorn, L., Drick, N., Pink, I., Dahlmann, J., Haase, A., Göhring, G., Welte, T., Martin, U., Ringshausen, F. C., and Olmer, R. 2020. Generation of two hiPSC clones (MHHi019-A, MHHi019-B) from a primary ciliary dyskinesia patient carrying a homozygous deletion in the NME5 gene (c.415delA (p.Ile139Tyrfs*8)). Stem Cell Res 48:101988.

https://doi.org/10.1016/j.scr.2020.101988.

 

Stelling, E., Ricke-Hoch, M., Erschow, S., Hoffmann, S., Bergmann, A. K., Heimerl, M., Pietzsch, S., Battmer, K., Haase, A., Stapel, B., Scherr, M., Balligand, J. L., Binah, O., and Hilfiker-Kleiner, D. 2020. Increased prostaglandin-D2 in male STAT3-deficient hearts shifts cardiac progenitor cells from endothelial to white adipocyte differentiation. PLoS Biol 18, no. 12:e3000739.

https://doi.org/10.1371/journal.pbio.3000739.

 

Szepes, M., Melchert, A., Dahlmann, J., Hegermann, J., Werlein, C., Jonigk, D., Haverich, A., Martin, U., Olmer, R., and Gruh, I. 2020. Dual Function of iPSC-Derived Pericyte-Like Cells in Vascularization and Fibrosis-Related Cardiac Tissue Remodeling In Vitro. Int J Mol Sci 21, no. 23.

https://doi.org/10.3390/ijms21238947.

 

Taghizadeh, S., Jones, M. R., Olmer, R., Ulrich, S., Danopoulos, S., Shen, C., Chen, C., Wilhelm, J., Martin, U., Chen, C., Al Alam, D., and Bellusci, S. 2020. Fgf10 Signaling-Based Evidence for the Existence of an Embryonic Stage Distinct From the Pseudoglandular Stage During Mouse Lung Development. Front Cell Dev Biol 8:576604.

https://doi.org/10.3389/fcell.2020.576604.

 

Weber, N.*, Kowalski, K.*, Holler, T., Radocaj, A., Fischer, M., Thiemann, S., de la Roche, J., Schwanke, K., Piep, B., Peschel, N., Krumm, U., Lingk, A., Wendland, M., Greten, S., Schmitto, J. D., Ismail, I., Warnecke, G., Zywietz, U., Chichkov, B., Meissner, J., Haverich, A., Martin, U., Brenner, B., Zweigerdt, R., and Kraft, T. 2020. Advanced Single-Cell Mapping Reveals that in hESC Cardiomyocytes Contraction Kinetics and Action Potential Are Independent of Myosin Isoform. Stem Cell Reports. * authors contributed equaly.

https://doi.org/10.1016/j.stemcr.2020.03.015.

 

Williams, B., Löbel, W., Finklea, F., Halloin, C., Ritzenhoff, K., Manstein, F., Mohammadi, S., Hashemi, M., Zweigerdt, R., Lipke, E., and Cremaschi, S. 2020. Prediction of Human Induced Pluripotent Stem Cell Cardiac Differentiation Outcome by Multifactorial Process Modeling. Front Bioeng Biotechnol 8:851.

https://doi.org/10.3389/fbioe.2020.00851.