Precision-cut liver slices as an ex vivo model for mixed liver tissue-lymphatic cell cultures

In this project, the organoid-like structure of precision-cut liver slices is used to establish an ex vivo organ-/immune-system interface simulating immune responses after engraftment of human donor livers. Precision-cut slices of donor livers performed prior engraftment serve as a three-dimensional in vitro liver culture system. Peripheral lymphocytes of the respective recipient are co-cultured with the donor liver tissue resembling a Mixed Liver Tissue / Lymphocyte Culture. Analyses of the liver tissue and the supernatant including flow cytometry and Luminex-based multiplex assays will be used to determine the immunological reactions, especially with respect to the differentiation of NK- and T-cell lymphocyte subsets.

Team

Dr. med. Nora Nevermann

PD Dr. med. Oliver Beetz

Dr. med. Philipp Tessmer

Dr. med. Clara Weigle

cand. med. Annika Liebsch

Funding

Transplant Center, Medical School Hannover