Analytical Ultracentrifugation
Structural Biochemistry operates a Beckman Coulter Optima AUC analytical ultracentrifuge, equipped with a UV/VIS absorbance and a Rayleigh interference optical system and a Beckman Coulter ProteomeLab XL-I equipped with an Aviv Biomedical fluorescence detection system.
Analytical ultracentrifugation can provide the following information about macromolecules
- molar mass (oligomerisation state)
- size and shape
- homogeneity
- protein-protein, protein-DNA, protein-ligand interactions
- determination of affinity constants of homo- and hetero-interactions
- investigation of fluorescently labeled proteins in complex mixtures like serum
- accessible concentration range from millimolar to nanomolar due to use of different optical systems
Contact: Prof. Dr. Ute Curth, curth.ute@mh-hannover.de, phone +49 511 532 9372
