Hammad R, Alzubi J, Rhiel M, Chmielewski KO, Mosti L, Rositzka J, Heugel M, Lawrenz J, Pennucci V, Gläser B, Fischer J, Schambach A, Moritz T, Lachmann N, Cornu TI, Mussolino C, Schäfer R, Cathomen T. “CRISPR-Cas12a for Highly Efficient and Marker-Free Targeted Integration in Human Pluripotent Stem Cells” Int J Mol Sci. 2024 Jan 12;25(2):985.  doi: 10.3390/ijms25020985

 

Abdin SM, Paasch D, Lachmann N. CAR macrophages on a fast track to solid tumor therapy. Nat Immunol. 2024 Jan;25(1):11-12. doi: 10.1038/s41590-023-01696-7 . PMID: 38168956

 

Rodriguez Gonzalez C, Schevel H, Hansen G, Schwerk N, Lachmann N. „Pulmonary Alveolar Proteinosis and new therapeutic concepts” Klin Padiatr. 2024 Jan 29. https://doi.org/10.1055/a-2233-1243

 

Paasch D, Lachmann N, “CAR macrophages tuning the immune symphony of anti-cancer therapies” Cell Stem Cell. 2024 DOI:https://doi.org/10.1016/j.stem.2024.05.006

 

Jahn C, Juchem M, Sonnenschein K, Gietz A, Buchegger T, Lachmann N, Göhring G, Behrens YL, Bär C, Thum T, Hoepfner J. „Generation of human induced pluripotent stem cell line MHHi029-A from a male Fabry disease patient carrying c.959A > T mutation“ Stem Cell Res. 2024 Mar 24:77:103404.  doi: 10.1016/j.scr.2024.103404

 

Pecksen E, Tkachuk S, Schröder C, Vives Enrich M, Neog A, Johnson CP, Lachmann N, Haller H, Kiyan Y. “Monocytes prevent apoptosis of iPSCs and promote differentiation of kidney organoids” Stem Cell Res Ther. 2024 May 3;15(1):132. doi: 10.1186/s13287-024-03739-8.

 

Abdin SM, Mansel F, Hashtchin AR, Ackermann M, Hansen G, Becker B, Kick B, Pham N, Dietz H, Schaniel C, Martin U, Spreitzer I, Lachmann N.„Sensor macrophages derived from human induced pluripotent stem cells to assess pyrogenic contaminations in parenteral drugs.“ Biofabrication. 2024 May 17;16(3). doi: 10.1088/1758-5090/ad4744.

 

Neehus AL, Carey B, Landekic M, Panikulam P, Deutsch G, Ogishi M, Arango-Franco CA, Philippot Q, Modaresi M, Mohammadzadeh I, Corcini Berndt M, Rinchai D, Le Voyer T, Rosain J, Momenilandi M, Martin-Fernandez M, Khan T, Bohlen J, Han JE, Deslys A, Bernard M, Gajardo-Carrasco T, Soudée C, Le Floc'h C, Migaud M, Seeleuthner Y, Jang MS, Nikolouli E, Seyedpour S, Begueret H, Emile JF, Le Guen P, Tavazzi G, Colombo CNJ, Marzani FC, Angelini M, Trespidi F, Ghirardello S, Alipour N, Molitor A, Carapito R, Mazloomrezaei M, Rokni-Zadeh H, Changi-Ashtiani M, Brouzes C, Vargas P, Borghesi A, Lachmann N, Bahram S, Crestani B, Pahari S, Schlesinger LS, Marr N, Bugonovic D, Boisson-Dupuis S, Béziat V, Abel L, Borie R, Young LR, Deterding R, Shahrooei M, Rezaei N, Parvaneh N, Craven D, Gros P, Malo D, Sepulveda FE, Nogee LM, Aladjidi N, Trapnell BC, Casanova JL, Bustamante J. “Human inherited CCR2 deficiency underlies progressive polycystic lung disease.” Cell. 2024 May 21:S0092-8674(24)00530-0. doi: 10.1016/j.cell.2024.05.021.

 

Momenilandi M, Lévy R, Sobrino S, Li J, Lagresle-Peyrou C, Esmaeilzadeh H, Fayand A, Le Floc'h C, Guérin A, Mina ED, Shearer D, Delmonte OM, Yatim A, Mulder K, Mancini M, Rinchai D, Denis A, Neehus AL, Balogh K, Brendle S, Rokni-Zadeh H, Changi-Ashtiani M, Seeleuthner Y, Deswarte C, Bessot B, Cremades C, Materna M, Cederholm A, Ogishi M, Philippot Q, Beganovic O, Ackermann M, Wuyts M, Khan T, Fouéré S, Herms F, Chanal J, Palterer B, Bruneau J, Molina TJ, Leclerc-Mercier S, Prétet JL, Youssefian L, Vahidnezhad H, Parvaneh N, Claeys KG, Schrijvers R, Luka M, Pérot P, Fourgeaud J, Nourrisson C, Poirier P, Jouanguy E, Boisson-Dupuis S, Bustamante J, Notarangelo LD, Christensen N, Landegren N, Abel L, Marr N, Six E, Langlais D, Waterboer T, Ginhoux F, Ma CS, Tangye SG, Meyts I, Lachmann N, Hu J, Shahrooei M, Bossuyt X, Casanova JL, Béziat V. "FLT3L governs the development of partially overlapping hematopoietic lineages in humans and mice." Cell. 2024 May 23;187(11):2817-2837.e31. doi: 10.1016/j.cell.2024.04.009. Epub 2024 May 3.

 

Jacobsen C, Plückebaum N, Ssebyatika G, Beyer S, Mendes-Monteiro L, Wang J, Kropp KA, González-Motos V, Steinbrück L, Ritter B, Rodríguez-González C, Böning H, Nikolouli E, Kinchington PR, Lachmann N, Depledge DP, Krey T, Viejo-Borbolla A. Viral modulation of type II interferon increases T cell adhesion and virus spread. Nat Commun. 2024 Jun 22;15(1):5318. doi: 10.1038/s41467-024-49657-4.PMID: 38909022

 

Ackermann M, Saleh F, Abdin SM, Rafiei Hashtchin A, Gensch I, Golgath J, Carvalho Oliveira M, Nguyen AHH, Gaedcke S, Fenske A, Jang MS, Jirmo AC, Abeln M, Hansen G, Lachmann N. Standardized generation of human iPSC-derived hematopoietic organoids and macrophages utilizing a benchtop bioreactor platform under fully defined conditions.Stem Cell Res Ther. 2024 Jun 18;15(1):171. doi: 10.1186/s13287-024-03785-2.PMID: 38886860

 

Guillet S, Lazarov T, Jordan N, Boisson B, Tello M, Craddock B, Zhou T, Nishi C, Bareja R, Yang H, Rieux-Laucat F, Lorenzo RIF, Dyall SD, Isenberg D, D'Cruz D, Lachmann N, Elemento O, Viale A, Socci ND, Abel L, Nagata S, Huse M, Todd Miller W, Casanova JL, Geissmann F. ACK1 and BRK non-receptor tyrosine kinase deficiencies are associated with familial systemic lupus and involved in efferocytosis.medRxiv [Preprint]. 2024 Jun 5:2024.02.15.24302255. doi: 10.1101/2024.02.15.24302255.PMID: 38883731

 

 

Rosain J, Neehus AL, Manry J, Yang R, Le Pen J, Daher W, Liu Z, Chan YH, Tahuil N, Türel Ö, Bourgey M, Ogishi M, Doisne JM, Izquierdo HM, Shirasaki T, Le Voyer T, Guérin A, Bastard P, Moncada-Vélez M, Han JE, Khan T, Rapaport F, Hong SH, Cheung A, Haake K, Mindt BC, Pérez L, Philippot Q, Lee D, Zhang P, Rinchai D, Al Ali F, Ahmad Ata MM, Rahman M, Peel JN, Heissel S, Molina H, Kendir-Demirkol Y, Bailey R, Zhao S, Bohlen J, Mancini M, Seeleuthner Y, Roelens M, Lorenzo L, Soudée C, Paz MEJ, González ML, Jeljeli M, Soulier J, Romana S, L'Honneur AS, Materna M, Martínez-Barricarte R, Pochon M, Oleaga-Quintas C, Michev A, Migaud M, Lévy R, Alyanakian MA, Rozenberg F, Croft CA, Vogt G, Emile JF, Kremer L, Ma CS, Fritz JH, Lemon SM, Spaan AN, Manel N, Abel L, MacDonald MR, Boisson-Dupuis S, Marr N, Tangye SG, Di Santo JP, Zhang Q, Zhang SY, Rice CM, Béziat V, Lachmann N, Langlais D, Casanova JL, Gros P, Bustamante J. Human IRF1 governs macrophagic IFN-γ immunity to mycobacteria. Cell. 2023 Feb 2;186(3):621-645.e33. doi: 10.1016/j.cell.2022.12.038. PMID: 36736301; PMCID: PMC9907019.

 

Kuschnerow P, Munder A, de Buhr N, Mörgelin M, Jirmo AC, Ackermann M, von Köckritz-Blickwede M, Tümmler B, Cramer N. Competitive survival of clonal serial Pseudomonas aeruginosa isolates from cystic fibrosis airways in human neutrophils. iScience. 2023 Mar 24;26(4):106475. doi: 10.1016/j.isci.2023.106475. PMID: 37096049; PMCID: PMC10122015.

 

Jürgens C, Ssebyatika G, Beyer S, Plückebaum N, Kropp KA, González-Motos V, Ritter B, Böning H, Nikolouli E, Kinchington PR, Lachmann N, Depledge DP, Krey T, Viejo-Borbolla A. Viral modulation of type II interferon increases T cell adhesion and virus spread. bioRxiv [Preprint]. 2023 May 26:2023.05.26.542397. doi: 10.1101/2023.05.26.542397. PMID: 37292914; PMCID: PMC10246016.

 

Forde AJ, Kolter J, Zwicky P, Baasch S, Lohrmann F, Eckert M, Gres V, Lagies S, Gorka O, Rambold AS, Buescher JM, Kammerer B, Lachmann N, Prinz M, Gross O, Pearce EJ, Becher B, Henneke P. "Metabolic rewiring tunes dermal macrophages in staphylococcal skin infection" Science Immunology 2023 Aug 11 DOI:10.1126/sciimmunol.adg3517

 

Shum IO, Merkert S, Malysheva S, Jahn K, Lachmann N, Verboom M, Frieling H, Hallensleben M, Martin U. An Improved Protocol for Targeted Differentiation of Primed Human Induced Pluripotent Stem Cells into HLA-G-Expressing Trophoblasts to Enable the Modeling of Placenta-Related Disorders. Cells. 2023 Aug 15;12(16):2070. doi: 10.3390/cells12162070. PMID: 37626882; PMCID: PMC10453333.

 

Hetzel M, Gensch I, Ackermann M, Lachmann N. Adaptation of Human iPSC-Derived Macrophages Toward an Alveolar Macrophage-Like Phenotype Post-Intra-Pulmonary Transfer into Murine Models. Methods Mol Biol. 2024;2713:463-479. doi: 10.1007/978-1-0716-3437-0_31. PMID: 37639142.

 

Dannenmann B, Klimiankou M, Oswald B, Solovyeva A, Mardan J, Nasri M, Ritter M, Zahabi A, Arreba-Tutusaus P, Mir P, Stein F, Kandabarau S, Lachmann N, Moritz T, Morishima T, Konantz M, Lengerke C, Ripperger T, Steinemann D, Erlacher M, Niemeyer CM, Zeidler C, Welte K, Skokowa J. iPSC modeling of stage-specific leukemogenesis reveals BAALC as a key oncogene in severe congenital neutropenia. Cell Stem Cell. 2023 Sep 7;30(9):1282. doi: 10.1016/j.stem.2023.07.015. Erratum for: Cell Stem Cell. 2021 May 6;28(5):906-922.e6. PMID: 37683606.

 

Malainou C, Abdin SM, Lachmann N, Matt U, Herold S. Alveolar macrophages in tissue homeostasis, inflammation, and infection: evolving concepts of therapeutic targeting. J Clin Invest. 2023 Oct 2;133(19):e170501. doi: 10.1172/JCI170501. PMID: 37781922; PMCID: PMC10541196.

 

Schweikert A, Kenny S, Oglesby I, Glasgow A, de Santi C, Gensch I, Lachmann N, Desroziers T, Fletcher C, Snijders D, Nathan N, Hurley K; COST Open-ILD Group Management Committee. An evaluation of an open access iPSC training course: "How to model interstitial lung disease using patient-derived iPSCs". Stem Cell Res Ther. 2023 Dec 20;14(1):377. doi: 10.1186/s13287-023-03598-9. PMID: 38124115; PMCID: PMC10734099.

 

Abdin SM, Paasch D, Kloos A, Oliveira MC, Jang MS, Ackermann M, Stamopoulou A, Mroch PJ, Falk CS, von Kaisenberg CS, Schambach A, Heuser M, Moritz T, Hansen G, Morgan M, Lachmann N. Scalable generation of functional human iPSC-derived CAR-macrophages that efficiently eradicate CD19-positive leukemia. J Immunother Cancer. 2023 Dec 22;11(12):e007705. doi: 10.1136/jitc-2023-007705. PMID: 38135346; PMCID: PMC10749073.

 

Neehus AL, Carey B, Landekic M, Panikulam P, Deutsch G, Ogishi M, Arango-Franco CA, Philippot Q, Modaresi M, Mohammadzadeh I, Corcini Berndt M, Rinchai D, Le Voyer T, Rosain J, Momenilandi M, Martin-Fernandez M, Khan T, Bohlen J, Han JE, Deslys A, Bernard M, Gajardo-Carrasco T, Soudée C, Le Floc'h C, Migaud M, Seeleuthner Y, Jang MS, Nikolouli E, Seyedpour S, Begueret H, Emile JF, Le Guen P, Tavazzi G, Colombo CNJ, Marzani FC, Angelini M, Trespidi F, Ghirardello S, Alipour N, Molitor A, Carapito R, Mazloomrezaei M, Rokni-Zadeh H, Changi-Ashtiani M, Brouzes C, Vargas P, Borghesi A, Lachmann N, Bahram S, Crestani B, Pahari S, Schlesinger LS, Marr N, Bugonovic D, Boisson-Dupuis S, Béziat V, Abel L, Borie R, Young LR, Deterding R, Shahrooei M, Rezaei N, Parvaneh N, Craven D, Gros P, Malo D, Sepulveda FE, Nogee LM, Aladjidi N, Trapnell BC, Casanova JL, Bustamante J. Human inherited CCR2 deficiency underlies progressive polycystic lung disease. Cell. 2023 Dec 20:S0092-8674(23)01323-5. doi: 10.1016/j.cell.2023.11.036. Epub ahead of print. PMID: 38157855.

 

 

 

Nikolouli E, Reichstein J, Hansen G, Lachmann N. "In vitro systems to study inborn errors of immunity using human induced pluripotent stem cells." Front Immunol. 2022 Nov 17;13:1024935. doi: 10.3389/fimmu.2022.1024935. eCollection 2022.

 

Waqas SF, Sohail A, Nguyen AHH, Usman A, Ludwig T, Wegner A, Malik MNH, Schuchardt S, Geffers R, Winterhoff M, Merkert S, Martin U, Olmer R, Lachmann N, Pessler F. "ISG15 deficiency features a complex cellular phenotype that responds to treatment with itaconate and derivatives." Clin Transl Med. 2022 Jul;12(7):e931. doi: 10.1002/ctm2.931.

 

Wunderlich S, Haase A, Merkert S, Jahn K, Deest M, Frieling H, Glage S, Korte W, Martens A, Kirschning A, Zeug A, Ponimaskin E, Göhring G, Ackermann M, Lachmann N, Moritz T, Zweigerdt R, Martin U."Targeted biallelic integration of an inducible Caspase 9 suicide gene in iPSCs for safer therapies." Mol Ther Methods Clin Dev. 2022 May 31;26:84-94. doi: 10.1016/j.omtm.2022.05.011. eCollection 2022 Sep 8.

 

Kloos D, Lachmann N. "Generation of Human iPSC from Small Volume Peripheral Blood Samples." Methods Mol Biol. 2022;2429:27-39. doi: 10.1007/978-1-0716-1979-7_3.

 

Paasch D, Meyer J, Stamopoulou A, Lenz D, Kuehle J, Kloos D, Buchegger T, Holzinger A, Falk CS, Kloth C, von Kaisenberg CS, Abken H, Schambach A, Lachmann N, Morgan M, Moritz T. "Ex Vivo Generation of CAR Macrophages from Hematopoietic Stem and Progenitor Cells for Use in Cancer Therapy" Cells. 2022 Mar 15;11(6):994. doi: 10.3390/cells11060994.

Ackermann M, Rafiei Hashtchin A, Manstein F, Carvalho Oliveira M, Kempf H, Zweigerdt R, Lachmann N. "Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors" Nat Protoc. 2022 Feb;17(2):513-539. doi: 10.1038/s41596-021-00654-7. Epub 2022 Jan 17.

Rafiei Hashtchin A, Fehlhaber B, Hetzel M, Manstein F, Stalp JL, Glage S, Abeln M, Zweigerdt R, Munder A, Viemann D, Ackermann M, Lachmann N. "Human iPSC-derived macrophages for efficient Staphylococcus aureus clearance in a murine pulmonary infection model" Blood Adv. 2021 Dec 14;5(23):5190-5201. doi: 10.1182/bloodadvances.2021004853.

 

Abdin SM, Paasch D, Morgan M, Lachmann N. "CARs and beyond: tailoring macrophage-based cell therapeutics to combat solid malignancies“ J Immunother Cancer. 2021 Aug;9(8):e002741.  doi: 10.1136/jitc-2021-002741.

 

Mucci A, Antonarelli G, Caserta C, Vittoria FM, Desantis G, Pagani R, Greco B, Casucci M, Escobar G, Passerini L, Lachmann N, Sanvito F, Barcella M, Merelli I, Naldini L, Gentner B. “Myeloid cell-based delivery of IFN-γ reprograms the leukemia microenvironment and induces anti-tumoral immune responses” EMBO Mol Med. 2021 Aug 30;e13598.  doi: 10.15252/emmm.202013598

 

Kumar S, Koenig J, Schneider A, Wermeling F, Boddul S, Theobald SJ, Vollmer M, Kloos D, Lachmann N, Klawonn F, Lienenklaus S, Talbot SR, Bleich A, Wenzel N, von Kaisenberg C, Keck J, Stripecke R. “In Vivo Lentiviral Gene Delivery of HLA-DR and Vaccination of Humanized Mice for Improving the Human T and B Cell Immune Reconstitution” Biomedicines. 2021 Aug 5;9(8):961.  doi: 10.3390/biomedicines9080961.

 

Janosz E, Hetzel M, Spielmann H, Tumpara S, Rossdam C, Schwabbauer M, Kloos D, von Kaisenberg C, Schambach A, Buettner FFR, Janciauskiene S, Lachmann N, Moritz T. „Pulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo“  Gene Ther. 2021 Jul 19.  doi: 10.1038/s41434-021-00269-3

 

Neehus AL, Moriya K, Nieto-Patlán A, Le Voyer T, Lévy R, Özen A, Karakoc-Aydiner E, Baris S, Yildiran A, Altundag E, Roynard M, Haake K, Migaud M, Dorgham K, Gorochov G, Abel L, Lachmann N, Dogu F, Haskologlu S, İnce E, El-Benna J, Uzel G, Kiykim A, Boztug K, Roderick MR, Shahrooei M, Brogan PA, Abolhassani H, Hancioglu G, Parvaneh N, Belot A, Ikinciogullari A, Casanova JL, Puel A, Bustamante J. “Impaired respiratory burst contributes to infections in PKCδ-deficient patients.” J Exp Med. 2021 Sep 6;218(9):e20210501. doi: 10.1084/jem.20210501.

 

Dahlke J, Schott JW, Vollmer Barbosa P, Klatt D, Selich A, Lachmann N, Morgan M, Moritz T, Schambach A. “Efficient Genetic Safety Switches for Future Application of iPSC-Derived Cell Transplants” J Pers Med. 2021 Jun 17;11(6):565. doi: 10.3390/jpm11060565.

 

Dannenmann B, Klimiankou M, Oswald B, Solovyeva A, Mardan J, Nasri M, Ritter M, Zahabi A, Arreba-Tutusaus P, Mir P, Stein F, Kandabarau S, Lachmann N, Moritz T, Morishima T, Konantz M, Lengerke C, Ripperger T, Steinemann D, Erlacher M, Niemeyer CM, Zeidler C, Welte K, Skokowa J. „iPSC modeling of stage-specific leukemogenesis reveals BAALC as a key oncogene in severe congenital neutropenia“ Cell Stem Cell. 2021 May 6;28(5):906-922.e6.  doi: 10.1016/j.stem.2021.03.023. Epub 2021 Apr 23.

 

Hetzel M, Ackermann M, Lachmann N. „Beyond "Big Eaters": The Versatile Role of Alveolar Macrophages in Health and Disease“ Int J Mol Sci. 2021 Mar 24;22(7):3308.  doi: 10.3390/ijms22073308.

 

Hoffmann D, Sens J, Brennig S, Brand D, Philipp F, Vollmer Barbosa P, Kuehle J, Steinemann D, Lenz D, Buchegger T, Morgan M, Falk CS, Klein C, Lachmann N, Schambach A. „Genetic Correction of IL-10RB Deficiency Reconstitutes Anti-Inflammatory Regulation in iPSC-Derived Macrophages“ J Pers Med. 2021 Mar 20;11(3):221. doi: 10.3390/jpm11030221.

 

Lachmann N. “Makrophagen in der Krankheitsentstehung und Therapie”. BIOspektrum, 27(2), 149-151. DOI: 10.1007/s12268-021-1547-y

 

Mass E, Lachmann N. “From macrophage biology to macrophage-based cellular immunotherapies.” Gene Ther. 2021 Feb 4. doi: 10.1038/s41434-021-00221-5. Online ahead of print.

 

Ackermann M, Mucci A, McCabe A, Frei S, Wright K, Snapper SB, Lachmann N, Williams DA, Brendel C. “Restored macrophage function ameliorates disease pathophysiology in a mouse model for IL10 receptor deficient very early onset inflammatory bowel disease.” J Crohns Colitis. 2021 Feb 17:jjab031. doi: 10.1093/ecco-jcc/jjab031. Online ahead of print.

Ackermann M, Haake K, Kempf H, Kaschutnig P, Weiss AC, Nguyen AHH, Abeln M, Merkert S, Kühnel MP, Hartmann D, Jonigk D, Thum T, Kispert A, Milsom MD, Lachmann N. “A 3D iPSC-differentiation Model Identifies interleukin-3 as a Regulator of Early Human Hematopoietic Specification” Haematologica. 2020 Apr 23:haematol.2019.228064. doi: 10.3324/haematol.2019.228064

 

Drutman SB, Mansouri D, Mahdaviani SA, Neehus AL, Hum D, Bryk R, Hernandez N, Belkaya S, Rapaport F, Bigio B, Fisch R, Rahman M, Khan T, Al Ali F, Marjani M, Mansouri N, Lorenzo-Diaz L, Emile JF, Marr N, Jouanguy E, Bustamante J, Abel L, Boisson-Dupuis S, Béziat V, Nathan C, Casanova JL. „Fatal Cytomegalovirus Infection in an Adult With Inherited NOS2 Deficiency“ N Engl J Med. 2020 Jan 30;382(5):437-445.  doi: 10.1056/NEJMoa1910640.

 

Hahn K, Pollmann L, Nowak J, Nguyen AHH, Haake K, Neehus AL, Waqas SF, Pessler F, Baumann U, Hetzel M, Casanova JL, Schulz A, Bustamante J, Ackermann M, Lachmann N, “Human Lentiviral Gene Therapy Restores the Cellular Phenotype of Autosomal Recessive Complete IFN-γR1 Deficiency” Molecular Therapy Methods and Clinical Development 2020 [accepted]

 

Merkert S, Schubert M, Haase A, Janssens HM, Scholte B, Lachmann N, Göhring G, Martin U. “Generation of an Induced Pluripotent Stem Cell Line (MHHi018-A) From a Patient With Cystic Fibrosis Carrying p.Asn1303Lys (N1303K) Mutation” Stem Cell Res. 2020 Mar 25;44:101744. doi: 10.1016/j.scr.2020.101744. Online ahead of print.

 

Lipus A, Janosz E, Ackermann M, Hetzel M, Dahlke J, Buchegger T, Wunderlich S, Martin U, Cathomen T, Schambach A, Moritz T, Lachmann N. „Targeted Integration of Inducible Caspase-9 in Human iPSCs Allows Efficient in vitro Clearance of iPSCs and iPSC-Macrophages“ J Mol Sci, 21 (7)  2020 Apr 3 DOI: 10.3390/ijms21072481

 

Pongpamorn P, Dahlmann J, Haase A, Ebeling CT, Merkert S, Göhring G, Lachmann N, Martens A, Haverich A, Martin U, Olmer R “Generation of Three Induced Pluripotent Stem Cell Lines (MHHi012-A, MHHi013-A, MHHi014-A) From a Family With Loeys-Dietz Syndrome Carrying a Heterozygous p.M253I (c.759G>A) Mutation in the TGFBR1 Gene”  Stem Cell Res , 43, 101707  Mar 2020 DOI: 10.1016/j.scr.2020.101707

 

Hoffmann D, Kuehle J, Lenz D, Philipp F, Zychlinski D, Lachmann N, Moritz T, Steinemann D, Morgan M, Skokowa J, Klein C, Schambach A „Lentiviral Gene Therapy and Vitamin B3 Treatment Enable Granulocytic Differentiation of G6PC3-deficient Induced Pluripotent Stem Cells“ Gene Ther 2020 Feb 12 [Online ahead of print] DOI: 10.1038/s41434-020-0127-y

 

Haake K, Neehus AL, Buchegger T, Kühnel MP, Blank P, Philipp F, Olega-Quintas C, Schulz A, Grimley M, Goethe R, Jonigk D, Kalinke U, Boisson-Dupuis S, Casanova JL, Bustamante J, Lachmann N “Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway” Cells, 9 (2) 2020 Feb 19 DOI: 10.3390/cells9020483

 

Haake K, Wüstefeld T, Merkert S, Lüttge D, Göhring G, Auber B, Baumann U, Lachmann N, “Human STAT1 Gain-Of-Function iPSC Line From a Patient Suffering From Chronic Mucocutaneous Candidiasis” Stem Cell Res, 43, 101713  2020 Jan 17 [Online ahead of print]

Haake K, Lachmann N, “New Drugs for an Old Foe: Mycobacterium Tuberculosis Meets PSC-Derived Macrophages” Stem Cell Reports 13 (6), 957-959  2019 Dec 10 DOI: 10.1016/j.stemcr.2019.11.006

 

Bernecker C, Ackermann M, Lachmann N, Rohrhofer L, Zaehres H, Araúzo-Bravo MJ, van den Akker E, Schlenke P, Dorn I. „Enhanced ex vivo generation of erythroid cells from human induced pluripotent stem cells in a simplified cell culture system with low cytokine support.“ Stem Cells Dev. 2019 Oct 9. doi: 10.1089/scd.2019.0132. [Epub ahead of print]

 

Arumugam P, Suzuki T, Shima K, McCarthy C, Sallese A, Wessendarp M, Ma Y, Meyer J, Black D, Chalk C, Carey B, Lachmann N, Moritz T, Trapnell BC. “Long-Term Safety and Efficacy of Gene-Pulmonary Macrophage Transplantation Therapy of PAP in Csf2ra-/- Mice. Mol Ther. 2019 Sep 4;27(9):1597-1611

 

Hetzel M, Lopez-Rodriguez E, Mucci A, Nguyen AHH, Suzuki T, Shima K, Buchegger T, Dettmer S, Rodt T, Bankstahl JP, Malik P, Knudsen L, Schambach A, Hansen G, Trapnell BC, Lachmann N*, Moritz T*. “Effective hematopoietic stem cell-based gene therapy in a murine model of hereditary pulmonary alveolar proteinosis.” Haematologica. 2019 Jul 9

 

Dannenmann B, Zahabi A, Mir P, Oswald B, Bernhard R, Klimiankou M, Morishima T, Schulze-Osthoff K, Zeidler C, Kanz L, Lachmann N, Moritz T, Welte K, Skokowa J. „Human iPSC-based model of severe congenital neutropenia reveals elevated UPR and DNA damage in CD34+ cells preceding leukemic transformation.“ Exp Hematol. 2019 Jan 4. pii: S0301-472X(19)30004-9.

 

Haake K, Ackermann M, Lachmann N. „Concise Review: Towards the Clinical Translation of Induced Pluripotent Stem Cell-Derived Blood Cells-Ready for Take-Off. Stem Cells Transl Med. Stem Cells Transl Med. 2019 Apr;8(4):332-339. doi: 10.1002/sctm.18-0134.

 

Beneke V*, Küster F*, Neehus AL, Hesse C, Lopez-Rodriguez E, Haake K, Rafiei Hashtchin A, Schott JW, Walter D, Braun A, Wolkers WF, Ackermann M, Lachmann N. „An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages.“ Sci Rep. 2018 Nov 2;8(1):16281. doi: 10.1038/s41598-018-34524-2.

 

Ackermann M*, Kempf H*, Hetzel M, Hesse C, Hashtchin AR, Brinkert K, Schott JW, Haake K, Kühnel MP, Glage S, Figueiredo C, Jonigk D, Sewald K, Schambach A, Wronski S, Moritz T, Martin U, Zweigerdt R, Munder A, Lachmann N „Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections.“, Nature Communications. 2018 Nov 30;9(1):5088. doi: 10.1038/s41467-018-07570-7.

 

Happle C*, Lachmann N*, Ackermann M, Wetzke M, Mirenska A, Göhring G, Thomay K, Mucci A, Glomb T, Suzuki T, Glage S, Dittrich-Breiholz O, Trapnell B, Moritz T, Hansen G “Pulmonary transplantation of human iPSC-derived macrophages ameliorates hereditary lung disease” Am J Respir Crit Care Med. 2018 Apr 13. doi: 10.1164/rccm.201708-1562OC

 

Mucci A, Lopez-Rodriguez E, Hetzel M, Liu S, Suzuki T, Happle C, Ackermann M, Kempf H, Hillje R, Kunkiel J, Janosz E, Brennig S, Glage S, Bankstahl JP, Dettmer S, Rodt T, Gohring G, Trapnell B, Hansen G, Trapnell C, Knudsen L, Lachmann N*, Moritz T*. „iPSC-Derived Macrophages Effectively Treat Pulmonary Alveolar Proteinosis in Csf2rb-Deficient Mice.“ Stem Cell Reports. 2018 Sep 11;11(3):696-710. doi: 10.1016/j.stemcr.2018.07.006.

 

Hetzel M, Mucci A, Blank P, Nguyen AHH, Schiller J, Halle O, Kühnel MP, Billig S, Meineke R, Brand D, Herder V, Baumgärtner W, Bange FC, Goethe R, Jonigk D, Förster R, Gentner B, Casanova JL, Bustamante J, Schambach A, Kalinke U, Lachmann N. „Hematopoietic stem cell gene therapy for IFNγR1 deficiency protects mice from mycobacterial infections.“ Blood. 2018 Feb 1;131(5):533-545. doi: 10.1182/blood-2017-10-812859.

 

Neehus AL, Lam J, Haake K, Merkert S, Schmidt N, Mucci A, Ackermann M, Schubert M, Happle C, Kühnel MP, Blank P, Philipp F, Goethe R, Jonigk D, Martin U, Kalinke U, Baumann U, Schambach A, Roesler J, Lachmann N “Impaired IFNg-Signaling and Mycobacterial Clearance in IFNgR1 Deficient Human iPSC-Derived Macrophages” Stem Cell Reports. 2018 Jan 9;10(1):7-16

 

Kuhn A, Ackermann M, Mussolino C, Cathomen T, Lachmann N, Moritz T. “TALEN-mediated functional correction of human iPSC-derived macrophages in context of hereditary pulmonary alveolar proteinosis.”

Sci Rep. 2017 Nov 9;7(1):15195.

 

Kunkiel J, Gödecke N, Ackermann M, Hoffmann D, Schambach A, Lachmann N, Wirth D, Moritz T. “The CpG-sites of the CBX3 ubiquitous chromatin opening element are critical structural determinants for the anti-silencing function.” Sci Rep. 2017 Aug 11;7(1):7919

 

Hetzel M, Suzuki T, Hashtchin AR, Arumugam P, Carey B, Schwabbauer M, Kuhn A, Meyer J, Schambach A, Van Der Loo J, Moritz T, Trapnell BC, Lachmann N. “Function and Safety of Lentivirus-Mediated Gene Transfer for CSF2RA-Deficiency” Hum Gene Ther Methods. 2017 Dec;28(6):318-329

 

Ackermann M, Kuhn A, Kunkiel J, Merkert S, Martin U, Moritz T, Lachmann N “Ex vivo Generation of Genetically Modified Macrophages from Human Induced Pluripotent Stem Cells” Transfus Med Hemother 2017 Jun;44(3):135-142

 

Pittermann E, Lachmann N, MacLean G, Emmrich S, Ackermann M, Göhring G, Schlegelberger B, Welte K, Schambach A, Heckl D, Orkin SH, Cantz T, Klusmann JH. „Gene correction of HAX1 reversed Kostmann disease phenotype in patient-specific induced pluripotent stem cells.“ Blood Adv. 2017 Jun 2;1(14):903-914.

 

Lopez-Rodriguez E, Gay-Jordi G, Mucci A, Lachmann N, Serrano-Mollar A. “Lung surfactant metabolism: early in life, early in disease and target in cell therapy.” Cell Tissue Res. 2017 Mar;367(3):721-735

 

Hartmann D, Fiedler J, Sonnenschein K, Just A, Pfanne A, Zimmer K, Remke J, Foinquinos A, Butzlaff M, Schimmel K, Maegdefessel L, Hilfiker-Kleiner D, Lachmann N, Schober A, Froese N, Heineke J, Bauersachs J, Batkai S, Thum T. “MicroRNA-Based Therapy of GATA2-Deficient Vascular Disease. Circulation. 2016 Dec 13;134(24):1973-1990.

Mucci A, Kunkiel J, Suzuki T, Brennig S, Glage S, Kühnel MP, Ackermann M, Happle C, Kuhn A, Schambach A, Trapnell BC, Hansen G, Moritz T, Lachmann N „Murine iPSC-derived macrophages as a tool for disease modeling of hereditary Pulmonary Alveolar Proteinosis due to Csf2rb deficiency“ Stem Cell Reports. 2016 Aug 9;7(2):292-305

 

Börger AK, Eicke D, Wolf C, Gras C, Aufderbeck S, Schulze K, Engels L, Eiz-Vesper B, Schambach A, Guzman CA, Lachmann N, Moritz T, Martin U, Blasczyk R, Figueiredo C. „Generation of HLA-universal iPSCs-derived megakaryocytes and platelets for survival under refractoriness conditions.“ Mol Med 2016 May 6;22. doi: 10.2119/molmed.2015.00235. [Epub ahead of print]

 

Skuljec J, Cabanski M, Surdziel E, Lachmann N, Brennig S, Pul R, Jirmo AC, Habener A, Visic J, Dalüge K, Hennig C, Moritz T, Happle C, Hansen G. „Monocyte/macrophage lineage commitment and distribution are affected by the lack of regulatory T cells in scurfy mice.“ Eur J Immunol. 2016 Jul;46(7):1656-68

Brennig S, Lachmann N, Buchegger T, Hetzel M, Schambach A, Moritz T. „Chemoprotection of murine hematopoietic cells by combined gene transfer of cytidine deaminase (CDD) and multidrug resistance 1 gene (MDR1).“ J Exp Clin Cancer Res. 2015 Dec 12;34:148

 

Dreyer AK, Hoffmann D, Lachmann N, Ackermann M, Steinemann D, Timm B, Siler U, Reichenbach J, Grez M, Moritz T, Schambach A, Cathomen T. „TALEN-mediated functional correction of X-linked chronic granulomatous disease in patient-derived induced pluripotent stem cells.“ Biomaterials. 2015 Nov;69:191-200

 

Ackermann M, Liebhaber S, Klusmann JH, Lachmann N, „Lost in Translation: Pluripotent Stem Cell Derived Hematopoiesis“ EMBO Mol Med 2015 Jul 14;7(11):1388-402

 

Lachmann N*, Brennig S*, Hillje R, Schermeier H, Phaltane R, Dahlmann J, Gruh I, Heinz N, Schiedlmeier B, Baum C, Moritz T „Tightly regulated "all in one" lentiviral vectors for protection of human hematopoietic cells from anticancer chemotherapy“ Gene Therapy 2015 Nov;22(11):883-92*contributed equally

 

Lachmann N*, Czarnecki K*, Brennig S, Phaltane P, Heise M, Heinz N, Kempf H, Dilloo D, Kaever V, Schambach A, Heuser M, Moritz T “Deoxycytidine-kinase (dCK) knock-down as a novel myeloprotective strategy in the context of fludarabine, cytarabine, or cladribine therapy” Leukemia. 2015 Nov;29(11):2266-9 *contributed equally

 

Lachmann N*, Ackermann M*, Frenzel E, Liebhaber S, Brennig S, Happle C, Hoffmann D, Klimenkova O, Lüttge D, Buchegger T, Kühnel MP, Schambach A, Janciauskiene S, Figueiredo C, Hansen G, Skokowa J, Moritz T. „Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies“ Stem Cell Reports. 2015 Feb 10;4(2):282-96 *contributed equally

 

Müller-Kuller U*, Ackermann M*, Kolodziej S, Brendel C, Fritsch J, Lachmann N, Kunkel H, Lausen J, Schambach A, Moritz T, Grez M. „A minimal ubiquitous chromatin opening element (UCOE) effectively prevents silencing of juxtaposed heterologous promoters by epigenetic remodeling in multipotent and pluripotent stem cells“ Nucleic Acids Res. 2015 Feb 18;43(3):1577-92 *contributed equally

Suzuki T, Arumugam P, Sakagami T, Lachmann N, Chalk C, Sallese A, Abe S, Trapnell C, Carey B, Moritz T, Malik P, Lutzko C, Wood RE, Trapnell BC „ Pulmonary macrophage transplantation therapy“ Nature 2014 Oct 23;514(7523):450-4

 

Happle C*, Lachmann N*, Skuljec J, Wetzke M, Ackermann M, Brennig S, Mucci A, Jirmo AC, Groos S, Mirenska A, Hennig C, Rodt T, Bankstahl JP, Schwerk N, Moritz T, Hansen G. „Pulmonary transplantation of macrophage progenitors as effective and long-lasting therapy for hereditary pulmonary alveolar proteinosis„ Science Transl Med. 2014 Aug 20;6(250):250ra113. *contributed equally

 

Phaltane R, Lachmann N, Brennig S, Ackermann M, Modlich U, Moritz T. “Lentiviral MGMTP140K- mediated in vivo selection employing a ubiquitous chromatin opening element (A2UCOE) linked to a cellular promoter” Biomaterials. 2014 Aug;35(25):7204-13

 

Lachmann N*, Happle C*, Lüttge D, Wetzke M, Merkert S, Ackermann M, Kensah J, Jara-Avaca M, Mucci A, Skuljec J, Dittrich AM, Pfaff N, Brennig S, Schambach A, Steinemann D, Göhring G, Cantz T, Martin U, Schwerk N, Hansen G, Moritz T “Gene correction of human induced Pluripotent Stem Cells repairs the cellular phenotype in Pulmonary Alveolar Proteinosis” Am J Respir Crit Care Med. 2014 Jan 15;189(2):167-82 *contributed equally

 

Ackermann M, Lachmann N, Hartung S, Eggenschwiler R, Pfaff N, Happle C, Mucci A, Göhring G, Niemann H, Hansen G, Schambach A, Cantz T, Zweigerdt R, Moritz T “Promoter and lineage independent anti-silencing activity of the A2 ubiquitous chromatin opening element for optimized human pluripotent stem cell-based therapy” Biomaterials. 2014 Feb;35(5):1531-42

Abdin SM, Paasch D, Lachmann N. CAR macrophages on a fast track to solid tumor therapy. Nat Immunol. 2024 Jan;25(1):11-12. doi: 10.1038/s41590-023-01696-7. PMID: 38168956.