Wissenschaftliche Artikel in Fachzeitschriften

2025

• Patient-specific hiPSC-derived cardiomyocytes indicate allelic and contractile imbalance as pathogenic factor in early-stage Hypertrophic Cardiomyopathy, Natalie Weber, Judith Montag, Kathrin Kowalsk, Bogdan Iorga, Jeanne de la Roche, Tim Holler, Daniel Wojciechowski, Meike Wendland, Ante Radocaj, Anne-Kathrin Mayer, Anja Brunkhorst, Felix Osten, Valentin Burkart, Birgit Piep, Alea Bodenschatz, Pia Gibron, Kristin Schwanke, Annika Franke, Stefan Thiemann, Anastasia Koroleva, Angelika Pfanne, Maike Konsanke, Jan Fiedler, Jan Hegermann, Christoph Wredej, Christian Mühlfeld, Boris Chichkov, Martin Fischer, Thomas Thum, Antonio Francino, Ulrich Martin, Joachim Meißner, Robert Zweigerdt, Theresia Kraft, Journal of Molecular and Cellular Cardiology (JMCC) Volume 198, January 2025, Pages 112-125, https://doi.org/10.1016/j.yjmcc.2024.11.007; PubMed

2023

• Nonsense mediated decay factor UPF3B is associated with cMyBP-C haploinsufficiency in hypertrophic cardiomyopathy patients, Valentin Burkart, Kathrin Kowalski, Alina Disch, Denise Hilfiker-Kleiner , Sean Lal , Cristobal dos Remedios, Andreas Perrot, Andre Zeug, Evgeni Ponimaskin, Maike Kosanke, Oliver Dittrich-Breiholz, Theresia Kraft, Judith Montag, Journal of Molecular and Cellular Cardiology Volume 185 (2023) pages: 26–37, https://doi.org/10.1016/j.yjmcc.2023.09.008, Online ahead of print

2022

• Transcriptional bursts and heterogeneity among cardiomyocytes in hypertrophic cardiomyopathy, Valentin Burkart, Kathrin Kowalski, David Aldag-Niebling, Julia Beck, Dirk Alexander Frick, Tim Holler, Ante Radocaj, Birgit Piep, Andre Zeug, Denise Hilfiker-Kleiner, Cristobal G. dos Remedios, Jolanda van der Velden, Judith Montag and Theresia Kraft, Front. Cardiovasc. Med.,  August 2022, Sec. Cardiovascular Biologics and Regenerative Medicin, doi: 10.3389/fcvm.2022.987889

2020

• Advanced Single-Cell Mapping Reveals that in hESC Cardiomyocytes Contraction Kinetics and Action Potential Are Independent of Myosin Isoform, Natalie Weber, Kathrin Kowalski, Tim Holler, Ante Radocaj, Martin Fischer, Stefan Thiemann, Jeanne de la Roche, Kristin Schwanke, Birgit Piep, Neele Peschel, Uwe Krumm, Alexander Lingk, Meike Wendland, Stephan Greten, Jan Dieter Schmitto, Issam Ismail, Gregor Warnecke, Urs Zywietz, Boris Chichkov, Joachim Meißner, Axel Haverich, Ulrich Martin, Bernhard Brenner, Robert Zweigerdt, Theresia Kraft, stem cell report, Published:April 16, 2020, DOI: https://doi.org/10.1016/j.stemcr.2020.03.015, Journal free

2018

• Burst-Like Transcription of Mutant and Wildtype MYH7 -Alleles as Possible Origin of Cell-to-Cell Contractile Imbalance in Hypertrophic Cardiomyopathy, Montag, J., Kowalski, K., Makul, M., Ernstberger, P., Radocaj, A., Beck, J., Becker, E., Tripathi, S., Keyser, B., Mühlfeld, C., Wissel, K., Pich, A., van der Velden, J., dos Remedios, C. G., Perrot, A., Francino, A., Navarro-López, F., Brenner, B., & Kraft, T. (2018). Front. Physiol. 9, 1–15. doi:10.3389/fphys.2018.00359

Veröffentlichte Abstracts für Vorträge 

Burkart V., Kowalski K., Holler T., Hilfiker-Kleiner D., Lal S., dos Remedios C. G., Perrot A., Ho C., Dittrich-Breiholz O., Kraft T., Montag J., MYBPC3-haploinsufficiency in hypertrophic cardiomyopathy patients is associated with nonsense mediated decay factor UBF3B. 2022, J Muscle Res Cell Motil

Montag J., Weber N., Kowalski K., Mayer A.-K., Iorga B., Brunkhorst A., Zweigerdt R., Kraft T., Human pluripotent stem cell-derived cardiomyocytes mimic intercellular heterogeneity as pathomechanism in Hypertrophic Cardiomyopathy, 2022, Acta Physiologica, Vol.236, Issue S725

Burkart V., Kowalski K., Holler T., Hilfiker-Kleiner D., Lal S., dos Remedios C. G., Perrot A., Seidman C. E., Kosanke M., Kraft T., Montag J., Alterations of sarcomeric stoichiometry by nonsense mediated decay of MYBPC3-mRNA in hypertrophic cardiomyopathy patients with truncation mutations. 2022, Biophysical Journal, Vol.121, Issue 3, Suppl., 1, 435a

Kowalski K., Mayer A.-K., Piep B., Zweigerdt R., Schwanke K., Kraft T., Montag J. Burst-like transcription of MYH7 in Hypertrophic Cardiomyopathy patients is mimicked in patient-derived hPSC-CMs; Acta Biochimica Polonica, Vol. 68 No. S1, 57; 2021

Burkart V., Kowalski K., Beck J., Aldag-Niebling D., Radocaj A., Keyser B., van der Velden J., dos Remedios C., Hilfiker-Kleiner D., Kraft T., Montag J. Burst-like transcription of sarcomeric genes in hypertrophic cardiomyopathy. 2021, Biophysical Society Journal, Vol. 120, Issue 3, Suppl. 1, 103a 

Weber N.*, Kowalski K.*, Holler T., Radocaj A., Fischer M., de la Roche J., Thiemann S., Schwanke K., Lingk A., Krumm U., Piep B., Martin U., Zweigerdt R., Brenner B., Kraft T., In human embryonic stem cell-derived cardiomyocytes twitsch kinetics, action potential parameters and MyH-mRNA fractions are independent of the expressed myosin heavy chain isoform, 2019, Biophysical Society Journal, Vol.116, Issue 3, Suppl. 1, 118A * gleichwertiger Beitrag der Autoren

Kowalski K., Radocaj A., Francino A., Navarro-López F., dos Remedios C. G., Kraft T., Brenner B. Detection of transcription sites in FHC patients indicating a burst-like transcription concept for MYH7. 2017, Acta Physiol. 219 (S711):47

Radocaj A., Kowalski K., Montag J., Kraft T., Brenner B. Independent Stochastic Burst-like Transcription of Mutant and Wildtype Alleles as Mechanism for Cell-to-Cell Functional Imbalance in Hypertrophic Cardiomyopathy. 2017, Biophysical Journal, Vol. 112, Issue 3, p164a

Kraft T., Kowalski K., Montag J., Radocaj A., Makul M., Ernstberger P., Perrot A., Francino A., Navarro-López F., Brenner B. Hypertrophic Cardiomyopathy: Random burst-like transcription of wildtype and mutated allele as trigger for phenotype development. 2016, Circulation, Vol. 134, Issue suppl_1

Veröffentlichte Abstracts für Poster

Kowalski K., Riesselmann J.N., Weber N., Piep B., Franke A., Martin U., Zweigerdt R., Kraft T.,  Presence of T3 reduces active transcription of MYBPC3 and TNNI3 in cardiomyocytes; 2022, J Muscle Res Cell Motil

Burkart V., Kowalski K., Beck J., Aldag-Niebling D., Holler T., Radocaj A., Keyser B., van der Velden J., Lal S., dos Remedios C., Hilfiker-Kleiner D., Kraft T., Montag J. Burst-like transcription of sarcomeric genes is associated with cell-to-cell allelic and functional heterogeneity in Hypertrophic Cardiomyopathy; Acta Biochimica Polonica, Vol 68 No. S1, 48; 2021

Kowalski K., Riesselmann J.N., Weber N., Piep B., Franke A., Martin U., Zweigerdt R., Kraft T., T3 reduces transcriptional activty of MYBPC3 and TNNI3. 2021, Acta Physiol.

Kowalski K., Riesselmann J.N., Weber N., Piep B., Franke A., Martin U., Zweigerdt R., Kraft T., Influence of T3 on active transcription of MYBPC3 and TNNI3. 2021, Biophysical Society Journal, Vol. 120, Issue 3, Suppl. 1, 136a

Burkart V., Beck J., Kowalski K., van der Velden J., dos Remedios C. G., Montag J., Kraft T., Unequal allelic expression of mutated cardiac troponin I from cell-to-cell may induce contractile imbalance in Hypertrophic Cardiomyopathy. 2020, Biophysical Society Journal, Vol.118, Issue 3, Suppl.1, 424a

Kowalski K., Weber N., Holler T., Radocaj A., Fischer M., de la Roche J., Thiemann S., Schwanke K., Piep K., Piep B., Lingk A., Krumm U., Martin U., Zweigerdt R., Brenner B., Kraft T., Twitch kinetics and action potential parameters are independent of expressed myosin heavy chain isoform in human embryonic stem cell-derived cardiomyocytes. 2019, Acta Physiol. 227 (S719):146

Burkart V., Beck J., Kowalski K., van der Velden J., dos Remedios C. G., Montag J., Kraft T., Allelic imbalance of TNNI3 trough burst-like transcription may lead to contractile imbalance in hypertrophic cardiomyopathy patients. 2019, J Muscle Res Cell Motil 40

Makul M., Radocaj A., Ernstberger P., Montag J., Kowalski K., Keyser B., Perrot A., dos Remedios C. G., Brenner B., Kraft T. Myosin and cMyBP-C-mutations in Hypertrophic cardiomyopathy: Variable effects on calcium sensitivity and contractile imbalance from cell-to-cell, 2019, Biophysical Society Journal, Vol.116, Issue 3, Suppl. 1, 262A

Weber N.*, Kowalski K.*, Holler T., Radocaj A., Schwanke K., Lingk A., Krumm U., Wendland M., Zywietz U., Chickov B., Ulrich M., Zweigerdt R., Brenner B., Kraft T. Human embryonic stem-cell derived cardiomyocytes: Single-Cell Mapping to relate twitch kinetics to myosin heavy chain protein and mRNA-expression, 2018, Biophysical Journal, Vol.114, Issue 3, p.549a * gleichwertiger Beitrag der Autoren

Kowalski K., Radocaj A., Francino A., Navarro-López F., dos Remedios C. G., Kraft T., Brenner B. Visualization of active transcription sites in human cardiomyocytes supports the concept of burst-like transcription of MYH7. 2017, Biophysical Journal Vol. 112, Issue 3, Supplement 1, 210a

Kowalski K., Radocaj A., Francino A., Navarro-López F., dos Remedios C. G., Kraft T., Brenner B. Analysis of active transcription sites in single human cardiomyocytes reveals burst-like transcription of MYH7. 2017, J Muscle Res Cell Motil 38:360

Radocaj A., Kowalski K., Montag J., Kraft T., Brenner B. Model of independent stochastic burst-like transcription can explain observed functional and transcriptional variability among cardiomyocytes from Hypertrophic Cardiomyopathy patients. 2017, J Muscle Res Cell Motil 38:362

Radocaj A., Kowalski K., Montag J., Kraft T., Brenner B. Independent, stochastic, burst-like transcription of mutant and wildtype MYH7-alleles. A mechanism for functional and transcriptional variability among HCM (hypertrophic cardiomyopathy) cardiomyocytes. 2017, Acta Physiol. 219(S711):132

Weber N., Kowalski K., Holler T., Lingk A., Radocaj A., Krumm U., Wendland M., Zywietz U., Chichkov B., Schwanke K., Martin U., Zweigerdt R., Brenner B., Kraft T. Singe cell mapping used to assign mRNA and protein expression of cardiac myosin heavy chain to twitch kinetics of the same human embryonic stem cell-derived cardiomyocyte. 2017, J Muscle Res Cell Motil 38:344