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vein endothelial cells (HUVECs) or ECs obtained from adult individuals (HAOECs and HSVECs). The early senescence of iPSC derivatives has been observed in current protocols and the underlying reason has not [...] ZFNs, TALENs and the CRISPR/Cas9 technology, straightforward and precise manipulation of the genome of human cells became possible. In contrast to ZFN- and TALEN-based methods, the power and attractiveness [...] applications. For instance, prime editing or base editing can be utilized to insert or correct point mutations, and a deactivated Cas9 (dCas9) can be fused to activators or repressors allowing for transcriptional
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