Medizinische Hochschule Hannover : Publications

Publications - Prof. Dr. Ute Curth

Publications

Funk, L. M., G. Poschmann, F. R. von Pappenheim, A. Chari, K. M. Stegmann, A. Dickmanns, M. Wensien, N. Eulig, E. Paknia, G. Heyne, E. Penka, A. R. Pearson, C. Berndt, T. Fritz, S. Bazzi, J. Uranga, R. A. Mata, M. Dobbelstein, R. Hilgenfeld, U. Curth and K. Tittmann (2024).
Multiple redox switches of the SARS-CoV-2 main protease in vitro provide opportunities for drug design.
Nat Commun 15(1): 411.
Oleshko, O., N. Vollack-Hesse, A. Tiede, J. Hegermann, U. Curth and S. Werwitzke (2023).
Von Willebrand factor modulates immune complexes and the recall response against factor VIII in a murine hemophilia A model.
Blood Adv 7(21): 6771-6781.
Richter, K. und U. Curth (2022).
Analytische Ultrazentrifugation
In: Kurreck, J., J.W. Engels und F. Lottspeich [Hrsg.]: Bioanalytik 4. Auflage. Berlin, Heidelberg: Springer Spektrum, 2022, S. 462-471
Wensien, M., F. R. von Pappenheim, L. M. Funk, P. Kloskowski, U. Curth, U. Diederichsen, J. Uranga, J. Ye, P. Fang, K. T. Pan, H. Urlaub, R. A. Mata, V. Sautner and K. Tittmann (2021).
A lysine-cysteine redox switch with an NOS bridge regulates enzyme function.
Nature 593(7859): 460-464.

Bogutzki, A. and U. Curth (2021). Analytical Ultracentrifugation for Analysis of Protein-Nucleic Acid Interactions.
Methods Mol Biol 2263: 397-421.

Naniima, P., E. Naimo, S. Koch, U. Curth, K. R. Alkharsah, L. J. Ströh, A. Binz, J. M. Beneke, B. Vollmer, H. Böning, E. M. Borst, P. Desai, J. Bohne, M. Messerle, R. Bauerfeind, P. Legrand, B. Sodeik, T. F. Schulz and T. Krey (2021).
Assembly of infectious Kaposi's sarcoma-associated herpesvirus progeny requires formation of a pORF19 pentamer.
PLoS Biol 19(11): e3001423.

Rai, A., J. P. Klare, P. Y. A. Reinke, F. Englmaier, J. Fohrer, R. Fedorov, M. H. Taft, I. Chizhov, U. Curth, O. Plettenburg and D. J. Manstein (2021).
Structural and Biochemical Characterization of a Dye-Decolorizing Peroxidase from Dictyostelium discoideum.
Int J Mol Sci 22(12).

Fiebig, T., Cramer, J. T., Bethe, A., Baruch, P., Curth, U., Führing, J. I., Buettner, F. F. R., Vogel, U., Schubert, M., Fedorov, R. and Mühlenhoff, M. (2020).
Structural and mechanistic basis of capsule O-acetylation in Neisseria meningitidis serogroup A.
Nat Commun 11(1), 4723.
Zhang, L., Lin, D., Sun, X., Curth, U., Drosten, C., Sauerhering, L., Becker, S., Rox, K., and Hilgenfeld, R. (2020)
Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved alpha-ketoamide inhibitors.
Science 368, 409-412.

Bogutzki, A., Naue, N., Litz, L., Pich, A. and Curth, U. (2019)
E. coli primase and DNA polymerase III holoenzyme are able to bind concurrently to a primed template during DNA replication.
Sci Rep, 9, 14460.

Bruhmann, S., Ushakov, D.S., Winterhoff, M., Dickinson, R.B., Curth, U. and Faix, J. (2017).
Distinct VASP tetramers synergize in the processive elongation of individual actin filaments from clustered arrays.
Proc Natl Acad Sci U S A, 114, E5815-E5824.

Krausze, J., Probst, C., Curth, U., Reichelt, J., Saha, S., Schafflick, D., Heinz, D.W., Mendel, R.R., and Kruse, T. (2017).
Dimerization of the plant molybdenum insertase Cnx1E is required for synthesis of the molybdenum cofactor.
Biochem J 474, 163-178.
Zumbragel, F.K., Machtens, D.A., Curth, U., Luder, C.G., Reubold, T.F., and Eschenburg, S. (2017).
Survivin does not influence the anti-apoptotic action of XIAP on caspase-9.
Biochem Biophys Res Commun 482, 530-535.
Vamosi, G., Mucke, N., Muller, G., Krieger, J.W., Curth, U., Langowski, J., and Toth, K. (2016).
EGFP oligomers as natural fluorescence and hydrodynamic standards.
Sci Rep 6, 33022.
Reubold, T. F., K. Faelber, N. Plattner, Y. Posor, K. Ketel, U. Curth, J. Schlegel, R. Anand, D. J. Manstein, F. Noe, V. Haucke, O. Daumke and S. Eschenburg (2015).
Crystal structure of the dynamin tetramer.
Nature 525(7569): 404-408.
Lee, C., Wigren, E., Trcek, J., Peters, V., Kim, J., Hasni, M.S., Nimtz, M., Lindqvist, Y., Park, C., Curth, U., Lunsdorf, H., and Romling, U. (2015).
A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains.
Environ Microbiol 17, 4511-4526.
Zhao, H., R. Ghirlando, C. Alfonso, F. Arisaka, I. Attali, D. L. Bain, M. M. Bakhtina, D. F. Becker, G. J. Bedwell, A. Bekdemir, T. M. Besong, C. Birck, C. A. Brautigam, W. Brennerman, O. Byron, A. Bzowska, J. B. Chaires, C. T. Chaton, H. Colfen, K. D. Connaghan, K. A. Crowley, U. Curth, T. Daviter, W. L. Dean, A. I. Diez, C. Ebel, D. M. Eckert, L. E. Eisele, E. Eisenstein, P. England, C. Escalante, J. A. Fagan, R. Fairman, R. M. Finn, W. Fischle, J. G. de la Torre, J. Gor, H. Gustafsson, D. Hall, S. E. Harding, J. G. Cifre, A. B. Herr, E. E. Howell, R. S. Isaac, S. C. Jao, D. Jose, S. J. Kim, B. Kokona, J. A. Kornblatt, D. Kosek, E. Krayukhina, D. Krzizike, E. A. Kusznir, H. Kwon, A. Larson, T. M. Laue, A. Le Roy, A. P. Leech, H. Lilie, K. Luger, J. R. Luque-Ortega, J. Ma, C. A. May, E. L. Maynard, A. Modrak-Wojcik, Y. F. Mok, N. Mucke, L. Nagel-Steger, G. J. Narlikar, M. Noda, A. Nourse, T. Obsil, C. K. Park, J. K. Park, P. D. Pawelek, E. E. Perdue, S. J. Perkins, M. A. Perugini, C. L. Peterson, M. G. Peverelli, G. Piszczek, G. Prag, P. E. Prevelige, B. D. Raynal, L. Rezabkova, K. Richter, A. E. Ringel, R. Rosenberg, A. J. Rowe, A. C. Rufer, D. J. Scott, J. G. Seravalli, A. S. Solovyova, R. Song, D. Staunton, C. Stoddard, K. Stott, H. M. Strauss, W. W. Streicher, J. P. Sumida, S. G. Swygert, R. H. Szczepanowski, I. Tessmer, R. T. t. Toth, A. Tripathy, S. Uchiyama, S. F. Uebel, S. Unzai, A. V. Gruber, P. H. von Hippel, C. Wandrey, S. H. Wang, S. E. Weitzel, B. Wielgus-Kutrowska, C. Wolberger, M. Wolff, E. Wright, Y. S. Wu, J. M. Wubben and P. Schuck (2015).
A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation.
PLoS One 10(5): e0126420.
Ghirlando, R., H. Zhao, A. Balbo, G. Piszczek, U. Curth, C. A. Brautigam and P. Schuck (2014). Measurement of the temperature of the resting rotor in analytical ultracentrifugation.
Anal Biochem 458: 37-39.
Kuhle, K., J. Krausze, U. Curth, M. Rossle, K. Heuner, C. Lang and A. Flieger (2014).
Oligomerization inhibits Legionella pneumophila PlaB phospholipase A activity.
J Biol Chem 289(27): 18657-18666.
Naue, N., M. Beerbaum, A. Bogutzki, P. Schmieder and U. Curth (2013)
The helicase-binding domain of Escherichia coli DnaG primase interacts with the highly conserved C-terminal region of single-stranded DNA-binding protein.
Nucleic Acids Res. 41(8): 4507-4517
Zhao, H., R. Ghirlando, G. Piszczek, U. Curth, C. A. Brautigam and P. Schuck (2013)
Recorded Scan Times Can Limit the Accuracy of Sedimentation Coefficients in Analytical Ultracentrifugation.
Anal Biochem. 437(1): 104-108
Ringel, P., J. Krausze, J. van den Heuvel, U. Curth, A. J. Pierik, S. Herzog, R. R. Mendel and T. Kruse (2013).
Biochemical characterization of molybdenum cofactor-free nitrate reductase from Neurospora crassa.
J Biol Chem 288(20): 14657-14671.
Lakshminarasimhan, M., U. Curth, S. Moniot, S. Mosalaganti, S. Raunser and C. Steegborn (2013). Molecular architecture of the human protein deacetylase Sirt1 and its regulation by AROS and resveratrol.
Biosci Rep 33(3).
Naue, N. and U. Curth (2012)
Investigation of protein-protein interactions of single-stranded DNA-binding proteins by analytical ultracentrifugation.
Methods Mol. Biol. 922: 133-149.
Wyszomirski, K. H., U. Curth, J. Alves, P. Mackeldanz, E. Moncke-Buchner, M. Schutkowski, D. H. Kruger and M. Reuter (2012)
Type III restriction endonuclease EcoP15I is a heterotrimeric complex containing one Res subunit with several DNA-binding regions and ATPase activity.
Nucleic Acids Res. 40(8): 3610-3622
Fonfara I., Curth U., Pingoud A., Wende W. (2012).
Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases.
Nucleic Acids Res. 40(2):847-860
El Houry Mignan S., Witte G., Naue N., Curth U. (2011).
Characterization of the chi psi subcomplex of Pseudomonas aeruginosa DNA polymerase III.
BMC Mol Biol. 12(1):43.
Linkner, J., Witte, G., Stradal, T., Curth, U. and Faix, J. (2011)
High-Resolution X-Ray Structure of the Trimeric Scar/WAVE-Complex Precursor Brk1
PLoS One 6, e21327
Winkler, I., A. D. Marx, D. Lariviere, R. Heinze, M. Cristovao, A. Reumer, U. Curth, T. K. Sixma and P. Friedhoff (2011)
Chemical trapping of the dynamic MutS-MutL complex formed in DNA mismatch repair in Escherichia coli
J Biol Chem 286, 17326-17337
Breitsprecher, D., A. K. Kiesewetter, J. Linkner, M. Vinzenz, T. E. Stradal, J. V. Small, U. Curth, R. B. Dickinson and J. Faix (2011)
Molecular mechanism of Ena/VASP-mediated actin-filament elongation.
Embo J. 30: 456-467.
Naue, N., R. Fedorov, A. Pich, D. J. Manstein and U. Curth (2011)
Site-directed mutagenesis of the χ subunit of DNA polymerase III and single-stranded DNA-binding protein of E. coli reveals key residues for their interaction.
Nucleic Acids Res. 39(4): 1398-407
Hoffmann, A., P. N. Dannhauser, S. Groos, L. Hinrichsen, U. Curth and E. J. Ungewickell (2010)
A comparison of GFP-tagged clathrin light chains with fluorochromated light chains in vivo and in vitro
Traffic 11(9): 1129-40
Arad G., Hendel, A., Urbanke, C., Curth, U. and Livneh, Z. (2008)
Single-stranded DNA-binding protein recruits DNA polymerase V to primer termini on RecA-coated DNA
J. Biol. Chem, 283 (13), 8274-8282
Witte, G., Fedorov, R. and Curth, U. (2008)
Biophysical analysis of Thermus aquaticus single-stranded DNA binding protein
Biophys. J., 94 (6), 2269-79
Curth, U. und Urbanke, C. (2007)
Analytische Ultrazentrifugation: Charakterisierung von Protein-Protein Wechselwirkungen.
BIOspektrum 13 (6), 643-645
Fedorov, R., Witte, G., Urbanke, C., Manstein, D.J. and Curth, U. (2006)
3D structure of Thermus aquaticus single-stranded DNA-binding protein gives insight into the functioning of SSB proteins.
Nucleic Acids Res, 34, 6708-6717.
Urbanke, C., Witte, G. and Curth, U. (2005)
Sedimentation velocity method in the analytical ultracentrifuge for the study of protein-protein interactions.
Methods Mol Biol, 305, 101-114
Witte, G., C. Urbanke and U. Curth (2005)
Single-stranded DNA-binding protein of Deinococcus radiodurans: a biophysical characterization.
Nucl. Acids Res. 33 (5): 1662-1670.
Witte, G., C. Urbanke and U. Curth (2003)
DNA polymerase III chi subunit ties single-stranded DNA binding protein to the bacterial replication machinery.
Nucl. Acids. Res. 31(15): 4434-4440
Landwehr, M., U. Curth and C. Urbanke (2002)
A dimeric mutant of the homotetrameric single-stranded DNA binding protein from Escherichia coli.
Biol Chem 383(9): 1325-33
Genschel, J., Curth, U., Urbanke, C. (2000)
Interaction of E. coli single-stranded DNA binding protein (SSB) with exonuclease I. The carboxy-terminus of SSB is the recognition site for the nuclease
Biological Chemistry 381, 183-192
Carlini,L., Curth,U., Kindler,B., Urbanke,C., Porter,R.D. (1998)
Identification of amino acids stabilizing the tetramerization of the single stranded DNA binding protein from Escherichia coli
FEBS Letters 430, 197-200
Yang, C., Curth, U., Urbanke, C. and Kang, C.-H. (1997)
Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4Å resolution
Nature structural biology 4, 153
Webster, G., Genschel, J., Curth, U. Urbanke, C., Kang, C. and Hilgenfeld, R. (1997)
A common core for binding single-stranded DNA: Structural comparison of the single-stranded DNA-binding proteins from E. coli and human mitochondria
FEBS Letters 411, 313-316.
Curth, U., Genschel, J., Urbanke, C. and Greipel, J. (1996)
In vitro and in vivo function of the carboxyterminus of E. coli single-stranded DNA binding protein
Nucleic Acids Research 24, 2706-2711
Misselwitz, R., Welfle, K., Curth, U., Urbanke, C. and Welfle, H. (1995)
Stability of Escherichia coli single-stranded DNA binding protein (EcoSSB)
Journal of Biomolecular Structure and Dynamics 12, 1041-1054
Curth, U., Urbanke, C., Greipel, J., Gerberding, H., Tiranti, V. and Zeviani, M. (1994)
Single-stranded-DNA-binding proteins from human mitochondria and Escherichia coli have analogous physicochemical properties
European Journal of Biochemistry 221, 435-443
Carlini, L.E., Porter, R.D., Curth, U. and Urbanke, C. (1993)
Viability and preliminary in vivo characterization of site-directed mutants of Escherichia coli single-stranded DNA-binding protein
Mol.Microbiol. 10, 1067-1075
Curth, U., Greipel, J., Urbanke, C. and Maass, G. (1993)
Multiple binding modes of the single-stranded DNA binding protein from Escherichia coli as detected by tryptophan fluorescence and site-directed mutagenesis
Biochemistry 32, 2585-2591
Curth, U., Bayer, I., Greipel, J., Mayer, F., Urbanke, C. and Maass, G. (1991)
Amino Acid 55 plays a central role in tetramerization and function of E. coli single stranded DNA binding protein
Eur.J.Biochem. 196, 87-93